We studied the effectiveness of trilysine (Lys3), tetralysine (Lys4), pentalysine (Lys5), and poly-l-lysine (PLL) (MW 50000) on lambda-DNA nanoparticle formation and characterized the size, shape, and stability of nanoparticles. Light scattering experiments showed EC50 (lysine concentration at 50% DNA compaction) values of approximately 0.0036, 2, and 20 micromol/L, respectively, for PLL, Lys5, and Lys4 at 10 mM [Na+]. Plots of log EC50 versus log [Na+] showed positive slopes of 1.09 and 1.7, respectively, for Lys4 and Lys5 and a negative slope of -0.1 for PLL. Hydrodynamic radii of oligolysine condensed particles increased (48-173 nm) with increasing [Na+], whereas no significant change occurred to nanoparticles formed with PLL. There was an increase in the size of nanoparticles formed with Lys5 at >40 degrees C, whereas no such change occurred with PLL. The DNA melting temperature increased with oligolysine concentration. These results indicate distinct differences in the mechanism(s) by which oligolysines and PLL provoke DNA condensation to nanoparticles.

译文

我们研究了三赖氨酸 (Lys3),四赖氨酸 (Lys4),五赖氨酸 (Lys5) 和聚-l-赖氨酸 (PLL) (MW 50000) 对lambda-DNA纳米颗粒形成的有效性,并表征了纳米颗粒的大小,形状和稳定性。光散射实验显示,对于PLL,Lys5和Lys4在10 mM [Na +] 下的EC50 (50% DNA压实时的赖氨酸浓度) 值分别约为0.0036、2和20微摩尔/L。log EC50与log [Na] 的图分别显示Lys4和Lys5的正斜率为1.09和1.7,PLL的负斜率为-0.1。低聚赖氨酸缩合颗粒的流体力学半径随着 [Na] 的增加而增加 (48-173 nm),而PLL形成的纳米颗粒没有显着变化。在> 40摄氏度下,用Lys5形成的纳米颗粒的尺寸增加,而PLL没有发生这种变化。DNA熔化温度随低聚赖氨酸浓度的增加而增加。这些结果表明低聚物和PLL引发DNA缩合到纳米颗粒的机理存在明显差异。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录