Smart scaffolds have a great role in the damaged tissue reconstruction. The aim of this study was developing a scaffold that in addition to its fiber's topography has also content of micro-RNAs (miRNAs), which play a regulatory role during osteogenesis. In this study, we inserted two important miRNAs, including miR-22 and miR-126 in the electrospun polycaprolactone (PCL) nanofibers and after scaffold characterization, osteoinductivity of the fabricated nanofibers was investigated by evaluating of the osteogenic differentiation potential of induced pluripotent stem cells (iPSCs) when grown on miRNAs-incorporated PCL nanofibers (PCL-miR) and empty PCL. MiRNAs incorporation had no effect on the fibers size and morphology, cell attachment, and protein adsorption, although viability and proliferation rate of the human iPSCs were increased after a week in PCL-miR compared to the empty PCL. The results obtained from alkaline phosphatase activity, calcium content, bone-related genes, and proteins expression assays demonstrated that the highest osteogenic markers were observed in iPSCs grown on the PCL-miR compared to the cells cultured on PCL and culture plate. According to the results, miR-incorporated PCL nanofibers could be considered as a promising potential tissue-engineered construct for the treatment of patients with bone lesions and defects.

译文

智能支架在受损组织重建中起着重要作用。这项研究的目的是开发一种支架,该支架除了其纤维的形貌外,还具有微rna (mirna) 的含量,这些rna在成骨过程中起调节作用。在这项研究中,我们在电纺聚己内酯 (PCL) 纳米纤维中插入了两个重要的mirna,包括miR-22和miR-126,并在支架表征后,通过评估在结合有miRNAs的PCL纳米纤维 (PCL-miR) 和空PCL上生长时诱导多能干细胞 (ipsc) 的成骨分化潜力,研究了制造的纳米纤维的骨诱导性。MiRNAs掺入对纤维的大小和形态,细胞附着和蛋白质吸附没有影响,尽管与空PCL相比,pcl-mir一周后人iPSCs的活力和增殖率有所提高。从碱性磷酸酶活性,钙含量,骨相关基因和蛋白质表达分析获得的结果表明,与在PCL和培养板上培养的细胞相比,在PCL-miR上生长的iPSCs中观察到最高的成骨标记。根据结果,结合miR的PCL纳米纤维可以被认为是一种有前途的潜在组织工程构建体,用于治疗骨病变和缺损患者。

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