The purpose of this study was to synthesize a self-setting bioactive cement by incorporation of wollastonite nanofibers (WNFs) into calcium phosphate cement (CPC). The composition, morphology, setting time, compressive strength, hydrophilicity, and degradation of WNF-doped CPC (wnf-CPC) were investigated. Scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and inductively coupled plasma atomic emission spectroscopy were utilized. Additionally, methyl-thiazolyl-tetrazolium bromide assay, scanning electron microscopy, inductively coupled plasma atomic emission spectroscopy, and histological evaluation were used to study the cell and tissue responses to wnf-CPC, both in vitro and in vivo. The results confirmed that the addition of WNFs into CPC had no obvious effect on the setting time or the compressive strength of wnf-CPC, provided the WNF amount was not more than 10 wt%. However, the hydrophilicity and degradability of wnf-CPC were significantly improved by the addition of WNFs - this was because of the change of microstructure caused by the WNFs. The preferred dissolution of WNFs caused the formation of microporosity in wnf-CPC when soaked in tris hydrochloride solution. The microporosity enlarged the surface area of the wnf-CPC and so promoted degradation of the wnf-CPC when in contact with liquid. In addition, MG-63 cell attachment and proliferation on the wnf-CPC were superior to that on the CPC, indicating that incorporation of WNFs into CPC improved the biological properties for wnf-CPC. Following the implantation of wnf-CPC into bone defects of rabbits, histological evaluation showed that wnf-CPC enhanced the efficiency of new bone formation in comparison with CPC, indicating excellent biocompatibility and osteogenesis of wnf-CPC. In conclusion, wnf-CPC exhibited promising prospects in bone regeneration.

译文

这项研究的目的是通过将硅灰石纳米纤维 (WNFs) 掺入磷酸钙水泥 (CPC) 中来合成自固化的生物活性水泥。研究了WNF掺杂的CPC (wnf-CPC) 的组成,形态,凝固时间,抗压强度,亲水性和降解。利用扫描电子显微镜,傅立叶变换红外光谱,x射线衍射和电感耦合等离子体原子发射光谱。此外,还使用甲基-噻唑基-溴化四唑鎓测定,扫描电子显微镜,电感耦合等离子体原子发射光谱和组织学评估来研究细胞和组织对wnf-CPC的体外和体内反应。结果证实,在CPC中添加WNFs对wnf-CPC的凝结时间或抗压强度没有明显影响,前提是WNF含量不超过10 wt %。然而,通过添加WNFs,wnf-CPC的亲水性和可降解性得到了显着改善-这是由于WNFs引起的微观结构变化。当浸泡在tris盐酸盐溶液中时,WNFs的优选溶解导致wnf-CPC中形成微孔。微孔扩大了wnf-CPC的表面积,因此在与液体接触时促进了wnf-CPC的降解。此外,MG-63细胞在wnf-CPC上的附着和增殖优于CPC,表明将WNFs掺入CPC中改善了wnf-CPC的生物学特性。将wnf-CPC植入兔骨缺损后,组织学评估表明,与CPC相比,wnf-CPC增强了新骨形成的效率,表明wnf-CPC具有出色的生物相容性和成骨作用。总之,wnf-CPC在骨再生方面具有广阔的前景。

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