Developing biomaterial constructs that closely mimic the natural tissue microenvironment with its complex chemical and physical cues is essential for improving the function and reliability of implantable devices, especially those that require direct neural-electrical interfaces. Here we demonstrate that free-standing vertically aligned carbon nanofiber (VACNF) arrays can be used as a multifunctional 3-D brush-like nanoengineered matrix that interpenetrates the neuronal network of PC12 cells. We found that PC12 neuron cells cultured on VACNF substrates can form extended neural network upon proper chemical and biochemical modifications. The soft 3-D VACNF architecture provides a new platform to fine-tune the topographical, mechanical, chemical, and electrical cues at subcellular nanoscale. This new biomaterial platform can be used for both fundamental studies of material-cell interactions and the development of chronically stable implantable neural devices. Micropatterned multiplex VACNF arrays can be selectively controlled by electrical and electrochemical methods to provide localized stimulation with extraordinary spatiotemporal resolution. Further development of this technology may potentially result in a highly multiplex closed-loop system with multifunctions for neuromodulation and neuroprostheses.

译文

开发生物材料结构,以其复杂的化学和物理线索紧密模仿自然组织微环境,对于改善可植入设备的功能和可靠性至关重要,尤其是那些需要直接神经电接口的设备。在这里,我们证明了独立式垂直排列的碳纳米纤维 (VACNF) 阵列可以用作互连PC12细胞神经元网络的多功能3-D刷状纳米工程基质。我们发现,在VACNF底物上培养的PC12神经元细胞可以在适当的化学和生化修饰后形成扩展的神经网络。软3-D VACNF架构提供了一个新的平台,可以在亚细胞纳米级上微调地形,机械,化学和电线索。这种新的生物材料平台可用于材料-细胞相互作用的基础研究以及长期稳定的可植入神经设备的开发。可以通过电气和电化学方法选择性地控制微图案化的多重VACNF阵列,以提供具有非凡时空分辨率的局部刺激。该技术的进一步发展可能会导致高度多重的闭环系统,该系统具有用于神经调节和神经假体的多功能功能。

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