We aimed to provide a tissue repair material, which can be synthesized rapidly, using polymers mimicking the natural environment in the extra-cellular matrix and metals/minerals. The components should have the potential to be used in tissue repair and simultaneously, reducing the side-effects of the incorporated molecules. It is challenging to manage the dispersibility of ZnO NPs in common solutions like water. Here, we report a novel method for preparing highly dispersible suspensions of ZnO NPs. In contrast to those synthesized by conventional methods, microwave assisted method allowed synthesis of dispersible ZnO NPs and the incorporation of zinc/Iron oxides NPs within alginate and gum matrix (AG) in a short span of time providing high yield of the product. The nanoformulations were characterized for size, morphology, interaction of various chemicals used during their synthesis by transmissible electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy and energy dispersive X ray Spectrum. It was also evaluated for cytotoxicity and their effect on equine fibroblast cells. Microwave-assisted fabrication of zinc/iron oxides nanoparticles provided flowerlike morphology with good dispersibility and high yield in a short span of time. Our results revealed that ZnO NPs were more cytotoxic than AG ZnO NPs and doped AG Fe3O4 doped ZnO NPs at higher concentrations. Further metal nanoparticles capped with alginate/acacia with size range less than 100 nm demonstrated high stability, good biocompatibility, re-epithelization and enhanced mineralization in horse fibroblast cells.

译文

我们旨在提供一种组织修复材料,该材料可以使用模仿细胞外基质和金属/矿物质中的自然环境的聚合物快速合成。这些成分应具有用于组织修复的潜力,同时减少掺入分子的副作用。管理ZnO np在水等常见解决方案中的分散性具有挑战性。在这里,我们报告了一种制备ZnO np高分散性悬浮液的新方法。与通过常规方法合成的方法相反,微波辅助方法允许在短时间内合成可分散的ZnO np,并在藻酸盐和树胶基质 (AG) 中掺入锌/铁氧化物np,从而提供高收率的产品。通过透射电子显微镜,x射线衍射,傅立叶变换红外光谱和能量色散x射线光谱,对纳米制剂的尺寸,形态,合成过程中使用的各种化学物质的相互作用进行了表征。还评估了其细胞毒性及其对马成纤维细胞的影响。微波辅助制备锌/铁氧化物纳米颗粒可在短时间内提供具有良好分散性和高产率的花朵状形态。我们的结果表明,在较高浓度下,ZnO NPs比AG ZnO NPs和掺杂的AG Fe3O4掺杂的ZnO NPs更具细胞毒性。用尺寸范围小于100 nm的藻酸盐/阿拉伯胶覆盖的其他金属纳米颗粒在马成纤维细胞中表现出高稳定性、良好的生物相容性、再上皮化和增强的矿化作用。

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