A capillary electrophoretic (CE) method for screening of six tricyclic antidepressants: amitriptyline, nortriptyline, imipramine, desipramine, doxepin and nordoxepin, in human serum, has been developed. The drugs were separated in a bare fused silica capillary (50 microm i.d.) using the background electrolyte: 50 mM CAPSO (pH 9.54) in methanol/water with KCI addition. For increasing the method sensitivity, the sample concentration in the capillary (sample stacking) using pressure and electrokinetic injection has been applied. The standard addition method was used for calibration of the developed analytical procedure. The precision of the identification parameter (the relative migration time) and the quantitative parameter (the relative peak area) was within the range of 0.05-1.65 and 0.73-6.7 (RSD %), respectively. The detection limits were found to be 30 ng/mL for desipramine, 62.5 ng/mL for nortriptyline, and 50 ng/mL for remaining analytes.

译文

已开发出一种毛细管电泳 (CE) 方法,用于筛选人血清中的六种三环抗抑郁药: 阿米替林,去甲替林,丙咪嗪,地昔帕明,多塞平和去甲氧磷。使用背景电解质: 50 mmcapso (pH 9.54) 在甲醇/水中添加KCI,在裸露的熔融石英毛细管 (50微米内径) 中分离药物。为了提高方法的灵敏度,已经应用了使用压力和电动注射的毛细管中的样品浓度 (样品堆叠)。标准添加方法用于校准所开发的分析程序。识别参数 (相对迁移时间) 和定量参数 (相对峰面积) 的精度分别在0.05-1.65和0.73-6.7 (RSD %) 的范围内。发现地昔帕明的检出限为30 ng/mL,去甲替林的检出限为62.5 ng/mL,其余分析物的检出限为50 ng/mL。

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