Microinfusion of anticonvulsants into the perirhinal cortex through 1 guide cannula in each hemisphere only invades a small area of this seizure controlling site in rats exposed to soman. The purpose of the present study was to examine whether infusions made through 2 cannulas in each perirhinal cortex may produce more efficacious anticonvulsant action against soman intoxication than the use of 1 cannula only in rats infused with the ionotropic antagonists procyclidine and caramiphen or the metabotropic glutamate modulators DCG-IV and MPEP. The results showed that the mere presence of indwelling double cannulas caused proconvulsant effect in response to subsequent systemic administration of soman. Both the control and caramiphen groups with double cannulas had significantly shorter latencies to seizure onset than the corresponding groups with single cannula. Procyclidine resulted in anticonvulsant efficacy, even in rats with double cannulas. In rats that received twin infusions of DCG-IV or MPEP, the anticonvulsant impact was very high, inasmuch as a majority of the rats in each group was protected against seizure activity. Drugs possessing powerful anticonvulsant potency can apparently counteract the proconvulsant effect of double cannulas, and some can even gain enhanced anticonvulsant capacity when invading a larger area of the perirhinal cortex. Perirhinal EEG recordings (electrodes in indwelling cannulas) in a separate set of rats not exposed to soman or drugs showed no differences in basal electrical activity (total power 0.5-25Hz or the theta band 4-12Hz) between groups with single or double cannulas. The intrinsic excitability and synaptic connectivity of the perirhinal cortex may be associated with the proconvulsant impact observed in rats with double cannulas when exposed to soman.

译文

在暴露于梭曼的大鼠中,通过每个半球的1个引导套管将抗惊厥药微注入到周围皮层中,仅侵入该癫痫发作控制部位的一小部分。本研究的目的是检查通过每个周围皮层中的2根套管输注是否比仅在注入离子型拮抗剂procyclidine和caramiphen的大鼠中使用1根套管对梭曼中毒产生更有效的抗惊厥作用或代谢型谷氨酸调节剂dcg-iv和MPEP。结果表明,仅留置双套管的存在会对随后的梭曼全身给药产生惊厥作用。具有双插管的对照组和卡拉米芬组的癫痫发作发作的潜伏期均明显短于具有单插管的相应组。即使在双插管的大鼠中,Procyclidine也具有抗惊厥的功效。在接受两次dcg-iv或MPEP输注的大鼠中,抗惊厥作用非常高,因为每组中的大多数大鼠都受到了癫痫发作的保护。具有强大抗惊厥效力的药物显然可以抵消双插管的促惊厥作用,有些甚至可以在侵入较大区域的周围皮层时获得增强的抗惊厥能力。在未暴露于梭曼或药物的另一组大鼠中,脊髓周围脑电图记录 (留置插管中的电极) 显示,具有单或双插管的组之间的基础电活动 (总功率0.5-25Hz或 θ 带4-12Hz) 没有差异。暴露于梭曼时,双套管大鼠的固有兴奋性和突触连通性可能与观察到的惊厥冲击有关。

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