Xenotransplantation using pig organs has achieved survival times up to 195 days in pig orthotopic heart transplantation into baboons. Here we demonstrate that in addition to an improved immunosuppressive regimen, non-ischaemic preservation with continuous perfusion and control of post-transplantation growth of the transplant, prevention of transmission of the porcine cytomegalovirus (PCMV) plays an important role in achieving long survival times. For the first time we demonstrate that PCMV transmission in orthotopic pig heart xenotransplantation was associated with a reduced survival time of the transplant and increased levels of IL-6 and TNFα were found in the transplanted baboon. Furthermore, high levels of tPA-PAI-1 complexes were found, suggesting a complete loss of the pro-fibrinolytic properties of the endothelial cells. These data show that PCMV has an important impact on transplant survival and call for elimination of PCMV from donor pigs.

译文

使用猪器官的异种移植在猪原位心脏移植到狒狒中获得了长达195天的存活时间。在这里,我们证明,除了改进的免疫抑制方案,持续灌注的非缺血保存和移植后生长的控制外,预防猪巨细胞病毒 (PCMV) 的传播在实现长存活时间中起着重要作用。我们首次证明了原位猪心脏异种移植中PCMV的传播与移植的存活时间减少有关,并且在移植的狒狒中发现了IL-6和tnf α 水平的升高。此外,发现了高水平的tPA-PAI-1复合物,表明内皮细胞的促纤溶特性完全丧失。这些数据表明,PCMV对移植存活具有重要影响,并呼吁从供体猪中消除PCMV。

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