The evaluation of a recently introduced kit (Cortipac (R)) for measuring plasma cortisol by competitive protein binding technique without prior extraction of steroids from plasma, is presented. The blank value was less than 2.5 mug/100 ml, and the lowest measurable value was 2.5 mug/100 ml. The coefficients of variation within- and between-batch determinations were 8.64% and 9.21% respectively. By adding known amounts of cortisol to pre-extracted plasma a linear correlation between calculated and measured cortisol was obtained. In comparison with the double isotope derivate technique, a linear correlation was found. A high degree of cross-reaction was found with nearly all of 11 tested steroids. Due to the considerably higher plasma cortisol concentration in most clinical cond-tions, the lack of specificity may be of minor importance, but must be considered in evaluating increased plasma cortisol values. The mean plasma cortisol concentration in normal subjects (20.3 mug/100 ml) was equal to or slightly higher than found by other methods. The kit was found appropriate for routine clinical application, due to the easy, rapid and inespensive performance with an acceptable recovery and reliability, but it cannot entirely replace more specific methods.

译文

介绍了最近推出的试剂盒 (Cortipac (R)) 的评估,该试剂盒通过竞争性蛋白质结合技术测量血浆皮质醇,而无需事先从血浆中提取类固醇。空白值小于2.5马克杯/100毫升,最低可测值为2.5马克杯/100毫升。批次内和批次间测定的变异系数分别为8.64% 和9.21%。通过将已知量的皮质醇添加到预提取的血浆中,可以获得计算的皮质醇与测量的皮质醇之间的线性相关性。与双同位素衍生技术相比,发现了线性相关性。几乎所有11种测试的类固醇都发现高度交叉反应。由于在大多数临床条件下血浆皮质醇浓度相当高,缺乏特异性可能不太重要,但在评估血浆皮质醇值增加时必须考虑这一点。正常受试者 (20.3杯/100毫升) 的平均血浆皮质醇浓度等于或略高于通过其他方法发现的。由于易于,快速和快速的性能以及可接受的恢复和可靠性,该试剂盒被认为适合常规临床应用,但不能完全替代更具体的方法。

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