We evaluated the immunoglobulin class responsible for the protective activity in serum obtained from humans and rabbits after immunization with the R595 (Re chemotype) mutant of Salmonella minnesota. Whole serum obtained before immunization and the IgG and IgM fractions failed to protect mice against lethal challenge with viable Klebsiella pneumoniae or Morganella morganii or with Salmonella typhi lipopolysaccharide (LPS). The protective activity of postimmunization serum resided solely in IgM antibody, whereas IgG antibody exhibited no protective activity. IgM antibody to the Re mutant was protective against bacterial challenge with both test strains of bacteria and S. typhi LPS. IgM antibody, at approximately the same concentration present in postimmunization serum, increased the LD50 of K. pneumoniae from less than 8.0 x 10(2) to greater than 2.0 x 10(4). These findings indicate that commercially prepared human IgG with high titers of antibody to antigens of the core portion of LPS would have little clinical utility.

译文

我们评估了用明尼苏达州沙门氏菌的R595 (Re化学型) 突变体预防接种从人和兔子获得的血清中负责保护活性的免疫球蛋白类别。预防接种前获得的全血清以及IgG和IgM组分未能保护小鼠免受可行的肺炎克雷伯菌或摩根氏菌或伤寒沙门氏菌脂多糖 (LPS) 的致命攻击。免疫后血清的保护活性仅停留在IgM抗体中,而IgG抗体则没有保护活性。针对Re突变体的IgM抗体对细菌和伤寒链球菌LPS的测试菌株均具有抗细菌攻击的保护作用。在免疫后血清中存在的浓度大致相同的IgM抗体将肺炎克雷伯菌的LD50从小于8.0 × 10(2) 增加到大于2.0 × 10(4)。这些发现表明,商业制备的具有针对LPS核心部分抗原的高滴度抗体的人IgG几乎没有临床用途。

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