Keratin intermediate filaments (IFs) form cross-linked arrays to fulfill their structural support function in epithelial cells and tissues subjected to external stress. How the cross-linking of keratin IFs impacts the morphology and differentiation of keratinocytes in the epidermis and related surface epithelia remains an open question. Experimental measurements have established that keratinocyte spreading area is inversely correlated to the extent of keratin IF bundling in two-dimensional culture. In an effort to quantitatively explain this relationship, we developed a mathematical model in which isotropic cell spreading is considered as a first approximation. Relevant physical properties such as actin protrusion, adhesion events, and the corresponding response of lamellum formation at the cell periphery are included in this model. Through optimization with experimental data that relate time-dependent changes in keratinocyte surface area during spreading, our simulation results confirm the notion that the organization and mechanical properties of cross-linked keratin filaments affect cell spreading; in addition, our results provide details of the kinetics of this effect. These in silico findings provide further support for the notion that differentiation-related changes in the density and intracellular organization of keratin IFs affect tissue architecture in epidermis and related stratified epithelia.

译文

角蛋白中间丝 (IFs) 形成交联阵列,以在承受外部压力的上皮细胞和组织中实现其结构支持功能。角蛋白IFs的交联如何影响表皮和相关表面上皮中角质形成细胞的形态和分化仍然是一个悬而未决的问题。实验测量已经确定,如果在二维培养物中捆绑,角质形成细胞的扩散面积与角蛋白的程度成反比。为了定量解释这种关系,我们开发了一个数学模型,其中各向同性单元扩展被视为第一近似值。该模型中包括相关的物理特性,例如肌动蛋白突出,粘附事件以及细胞外围的薄片形成的相应响应。通过优化与扩散过程中角质形成细胞表面积随时间变化相关的实验数据,我们的模拟结果证实了交联角蛋白丝的组织和机械性能影响细胞扩散的概念; 此外,我们的结果提供了这种效应的动力学细节。这些计算机研究结果为以下观点提供了进一步的支持: 角蛋白IFs的密度和细胞内组织的分化相关变化会影响表皮和相关分层上皮的组织结构。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录