In this study, we have investigated the expression of 87 micro (mi)RNAs in activated CD4(+) T cells cultured in the presence or absence of the immunoregulatory molecule soluble HLA-G (sHLA-G). We observed (i) a decreased miR-451 expression and (ii) an increased miR-210 expression in sHLA-G-treated CD4(+) T cells. By transfecting CD4(+) T cells with miR-210 and miR-451 mimics or inhibitors, we found that sHLA-G-mediated modulation of these miRNAs was not related to sHLA-G-mediated inhibition of (i) proliferation and (ii) CXCR3 expression in CD4(+) T cells. Finally, we investigated the expression of 14 genes targeted by miR-210 or miR-451 in activated CD4(+) T cells, treated or not with sHLA-G. We observed an increased expression of OSR-1 (odd-skipped related 1) and HBP-1 (HMG-box transcription factor 1) and a decreased expression of CXCL16 (chemokine C-X-C motif ligand 16) and C11orf30 (chromosome 11 open reading frame 30) in sHLA-G-treated CD4(+) T cells. In conclusion, sHLA-G triggered a modulation of miRNA expression that may in turn modulate downstream gene expression, thus affecting CD4(+) T-cell function.

译文

在这项研究中,我们已经研究了在存在或不存在免疫调节分子可溶性hla-g (shla-g) 的情况下培养的活化的CD4 () T细胞中87个微 (mi) rna的表达。我们观察到 (i) sHLA中miR-451表达降低和 (ii) miR-210表达增加-通过用miR-210和miR-451模拟物或抑制剂转染CD4(+) T细胞,我们发现shla-g介导的这些mirna的调节与shla-g介导的抑制CD4(+) T细胞增殖和CXCR3表达无关。我们研究了miR-210或miR-451靶向的14个基因在活化的CD4(+) T细胞中的表达,用sHLA-G治疗或不治疗。我们观察到OSR-1 (奇数跳过相关1) 和HBP-1 (HMG-box转录因子1) 的表达增加,CXCL16 (趋化因子c-x-c基序配体16) 和C11orf30 (11号染色体开放阅读框30) 的表达减少shla-g处理的CD4(+) T细胞。总之,shla-g触发了miRNA表达的调节,进而可能调节下游基因表达,从而影响CD4 () T细胞功能。

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