The natural carotenoid crocetin has been reported to suppress phenotypes of an experimental myopia model in mice. We investigated the minimum effective dose to prevent myopia progression in a murine model. Three-week-old male mice (C57B6/J) were equipped with a -30 diopter (D) lens to induce myopia, and fed with normal chow, 0.0003%, or 0.001% of crocetin-containing chow. Changes in refractive errors and axial lengths (AL) were evaluated after three weeks. Pharmacokinetics of crocetin in the plasma and the eyeballs of mice was evaluated with specific high sensitivity quantitative analysis using liquid chromatography tandem mass spectrometry (LC-MS/MS) to determine the minimum effective dosage. A concentration of 0.001% of crocetin-containing chow showed a significant (p < 0.001) suppressive effect against both refractive and AL changes in the murine model. Meanwhile, there was no significant difference of AL change between the 0.0003% and the normal chow groups. The concentration of crocetin in the plasma and the eyeballs from mice fed with 0.001% crocetin-containing chow was significantly higher than control and 0.0003% crocetin-containing chow. In conclusion, we suggest 0.001% of crocetin-containing extract is the minimum effective dose showing a significant suppressive effect against both refractive and AL changes in the murine model.

译文

据报道,天然类胡萝卜素crocetin可以抑制小鼠实验性近视模型的表型。我们研究了在鼠模型中预防近视进展的最小有效剂量。三周大的雄性小鼠 (C57B6/J) 配备有-30屈光度 (D) 晶状体以诱发近视,并饲喂正常的chow,0.0003% 或0.001% 的含有藏红花的chow。三周后评估屈光不正和轴向长度 (AL) 的变化。使用液相色谱串联质谱 (lc-ms/MS) 通过特定的高灵敏度定量分析评估了藏红花在小鼠血浆和眼球中的药代动力学,以确定最小有效剂量。在小鼠模型中,含有crocetin的chow浓度的0.001% 对屈光和AL变化均显示出显着的 (p < 0.001) 抑制作用。同时,0.0003% 组与正常组之间的AL变化无显着差异。用含有0.001% 的crocetin的chow喂养的小鼠的血浆和眼球中crocetin的浓度显着高于对照组和0.0003% 的crocetin的chow。总之,我们建议0.001% 含有crocetin的提取物是最小有效剂量,在小鼠模型中显示出对屈光和AL变化的显着抑制作用。

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