Atopic dermatitis (AD) is a chronic inflammatory skin disease caused mainly by immune dysregulation. This study explored the anti-inflammatory and immunomodulatory effects of the Centellaasiatica ethanol extract (CA) on an AD-like dermal disorder. Treatment with CA inhibited the expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in a dose-dependent manner in inflammatory stimulated HaCaT cells by interferon-γ (IFN-γ) and TNF-α-triggered inflammation. Eight-week-old BALB/c mice treated with 2,4-dinitrochlorobenzene (DNCB) were used as a mouse model of AD. In AD induce model, we had two types treatment of CA; skin local administration (80 µg/cm2, AD+CA-80) and oral administration (200 mg/kg/d, AD+CA-200). Interestingly, the CA-treated groups exhibited considerably decreased mast cell infiltration in the ear tissue. In addition, the expression of IL-6 in mast cells, as well as the expression of various pathogenic cytokines, such as TNF-α, IL-4, IL-5, IL-6, IL-10, IL-17, iNOS, COX-2, and CXCL9, was reduced in both AD+CA-80 and AD+CA-200 groups. Collectively, our data demonstrate the pharmacological role and signaling mechanism of CA in the regulation of allergic inflammation of the skin, which supports our hypothesis that CA could potentially be developed as a therapeutic agent for AD.

译文

特应性皮炎 (AD) 是一种主要由免疫失调引起的慢性炎症性皮肤病。这项研究探讨了积雪草乙醇提取物 (CA) 对AD样皮肤疾病的抗炎和免疫调节作用。CA治疗以剂量依赖性方式抑制干扰素-γ (IFN-γ) 和TNF-α 触发的炎症刺激HaCaT细胞中interleukin-6 (IL-6) 和肿瘤坏死因子-α (TNF-α) 的表达。炎症。用2,4-二硝基氯苯 (DNCB) 处理的八周龄BALB/c小鼠用作AD的小鼠模型。在AD诱导模型中,我们有两种类型的CA治疗: 皮肤局部给药 (80 µ g/cm2,AD + CA-80) 和口服给药 (200 mg/kg/d,AD + CA-200)。有趣的是,CA处理的组在耳组织中的肥大细胞浸润明显减少。此外,在AD + CA-80和AD + CA-200组中,肥大细胞中IL-6的表达以及各种致病细胞因子 (如TNF-α,IL-4,IL-5,IL-6,IL-10,IL-17,iNOS,COX-2和CXCL9) 的表达均降低。总的来说,我们的数据证明了CA在调节皮肤过敏性炎症中的药理作用和信号传导机制,这支持了我们的假设,即CA可能被开发为AD的治疗剂。

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