Black yeast, Aureobasidium pullulans is extracellularly produced β-(1,3), (1,6)-D-glucan (β-glucan) under certain conditions. In this study, using Glycine max cv. Kurosengoku (Kurosengoku soybeans), the production of β-glucan through fermentation of A. pullulans was evaluated, and the effects of A. pullulans cultured fluid (AP-CF) containing β-glucan made with Kurosengoku soybeans (kAP-CF) on a human monocyte derived cell line, Mono Mac 6 cells were investigated. Concentration of β-glucan in kAP-CF reached the same level as normal AP-CF. An anti-angiogenic protein, Thrombospondin-1 (THBS1) was effectively induced after the stimulation with kAP-CF for comparison with AP-CF. The THBS1 is also induced after stimulation with hot water extract of Kurosengoku soybeans (KS-E), while the combined stimulation of β-glucan with KS-E more effectively induced THBS1 than that with KS-E alone. These results suggest effects of A. pullulans-produced β-glucan on the enhancement of Kurosengoku soybean-induced THBS1 expression.

译文

黑色酵母,短梗霉在一定条件下在细胞外产生 β-(1,3),(1,6)-D-葡聚糖 (β-葡聚糖)。在这项研究中,使用Glycine max cv。Kurosengoku (Kurosengoku大豆),通过发酵产生 β-葡聚糖的方法进行了评估,以及含有Kurosengoku大豆 (kap-cf) 制成的 β-葡聚糖的A.Pululans培养液 (ap-cf) 对人单核细胞衍生细胞系的影响,研究了Mono Mac 6细胞。Kap-cf中 β-葡聚糖的浓度达到与正常ap-cf相同的水平。与ap-cf相比,用kap-cf刺激后有效诱导了抗血管生成蛋白Thrombospondin-1 (THBS1)。用Kurosengoku大豆 (ks-e) 的热水提取物刺激后,也诱导了THBS1,而 β-葡聚糖与ks-e的联合刺激比单独使用ks-e更有效地诱导了THBS1。这些结果表明,支链淀粉产生的 β-葡聚糖对增强Kurosengoku大豆诱导的THBS1表达的影响。

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