Lindera obtusiloba Blume, a native plant of East Asia, has traditionally been used as a folk medicine for liver disease. We studied the in vitro antioxidant and in vivo hepatoprotective activities of a 70% ethanolic extract of L. obtusiloba (LOE) containing 62.9% quercitrin and 22.0% afzelin. LOE prevented tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in HepG2 cells. Along with its high antioxidant potency in vitro, our animal study confirmed that pretreatment with LOE (500 or 2000 mg/kg) for 7 days prior to a single dose of t-BHP (i.p.: 0.5 mmol/kg) significantly lowered the serum levels of alanine and aspartate aminotransferases. In addition, glutathione levels were increased in the liver, and lipid peroxidation levels were decreased in a dose-dependent manner. The histopathological examinations of rat livers showed that LOE significantly reduced the incidence of liver lesions induced by t-BHP. Therefore, we concluded that LOE has merit as a potent candidate to protect the liver against oxidative damage.

译文

东亚本地植物Lindera obtusiloba Blume传统上被用作肝病的民间药物。我们研究了含有62.9% 槲皮素和22.0% 阿夫泽林的70% obtusiloba (LOE) 乙醇提取物的体外抗氧化和体内保肝活性。LOE阻止了叔丁基过氧化氢 (t-BHP) 诱导的HepG2细胞的氧化损伤。连同其在体外的高抗氧化能力,我们的动物研究证实,在单剂量t-BHP (i.p.: 0.5 mmol/kg) 之前用LOE (500或2000 mg/kg) 预处理7天显着降低丙氨酸和天冬氨酸氨基转移酶的血清水平。此外,肝脏中谷胱甘肽水平升高,脂质过氧化水平降低,呈剂量依赖性。大鼠肝脏的组织病理学检查表明,LOE显着降低了t-BHP引起的肝脏病变的发生率。因此,我们得出结论,LOE作为保护肝脏免受氧化损伤的有效候选者具有优势。

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