Previous results from our laboratory (Bejar et al. 1985) indicated that a single injection in mouse pups of the antimitotic/mutagenic agent methylazoxymethanol at postnatal day 5 typically produces hypogranular cerebella with no changes in foliation, in contrast to the severe alterations observed after the more usual injection on the day of birth. Here we report that injection of a higher dose (30 mg/kg) of methylazoxymethanol, always at postnatal day 5, leads to the additional presence of a ectopic cell layer in adult cerebellum. Immunostaining with several antibodies recognizing cell specific proteins ruled out the possibility that these ectopic cells were glial and electron microscopy indicated that they were morphologically mature granule cells. In the molecular layer of other cerebellar areas and apparently unrelated with granule cell ectopia, ectopic Golgi epithelial cells were observed. The reason for the presence of these ectopic cells of different type in the molecular layer was discussed in relation with analogous ectopias obtained by other means.

译文

我们实验室的先前结果 (Bejar等人1985) 表明,与出生当天更常见的注射后观察到的严重变化相反,在出生后第5天在小鼠幼崽中单次注射抗有丝分裂/诱变剂甲基偶氮氧甲醇通常会产生低颗粒小脑,而叶面没有变化。在这里,我们报告说,总是在出生后第5天注射更高剂量 (30 mg/kg) 的甲基偶氮甲醇会导致成年小脑中额外存在异位细胞层。用几种识别细胞特异性蛋白的抗体进行免疫染色,排除了这些异位细胞为神经胶质的可能性,电子显微镜检查表明它们在形态上是成熟的颗粒细胞。在其他小脑区域的分子层中,明显与颗粒细胞异位无关,观察到异位的高尔基体上皮细胞。讨论了在分子层中存在这些不同类型的异位细胞的原因,并与通过其他方法获得的类似异位有关。

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