A Gram-negative bacterial strain designated LS2 isolated from Lahaul-Spiti valley of North India was shown to produce pink pigment while utilizing methanol as sole source of carbon and energy. Interestingly, pigment production was inducible in nature since the organism did not produce any pigment when grown on other carbon sources. Based on phenotypic and phylogenetic characterization the non-pigmented methylotroph was identified as a novel strain of Acinetobacter lwoffii MTCC 8288 (DQ144736). By means of spectral and mass analyses the pigment was characterized as bacterioruberin-like carotenoid molecule. Here, the carotenoid pigment may form an important part of the antioxidant defense mechanism against oxidative stress imparted by methanol. The methanol utilization pathway in strain LS2 was deciphered by showing the presence of functional methanol dehydrogenase and formaldehyde dehydrogenase genes. In addition, to investigate methanol induced physiological changes, comparative fatty acid profile was analysed and distinctive qualitative as well as quantitative differences in fatty acid content were observed. Therefore, we suggest that strain LS2 exhibiting such unique phenotypic property should be assigned a taxonomic position other than the pigmented and non-pigmented methylotrophs.

译文

从印度北部的Lahaul-Spiti山谷分离出的革兰氏阴性细菌菌株LS2被证明可以产生粉红色色素,同时利用甲醇作为唯一的碳和能源来源。有趣的是,色素的产生在自然界中是可诱导的,因为该生物在其他碳源上生长时不会产生任何色素。基于表型和系统发育特征,非色素甲基营养被鉴定为lwoffii不动杆菌MTCC 8288 (DQ144736) 的新型菌株。通过光谱和质量分析,将色素表征为类细菌素类胡萝卜素分子。在这里,类胡萝卜素可能构成抗氧化防御机制的重要组成部分,以抵抗甲醇赋予的氧化应激。通过显示功能性甲醇脱氢酶和甲醛脱氢酶基因的存在,破译了菌株LS2中的甲醇利用途径。此外,为了研究甲醇诱导的生理变化,分析了比较脂肪酸分布,并观察了脂肪酸含量的明显定性和定量差异。因此,我们建议表现出这种独特表型特性的LS2菌株应被指定为除色素和非色素甲基营养菌以外的分类学位置。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录