Healing of the rat Achilles tendon is sensitive to mechanical loading, and the callus strength is reduced by 3/4 after 14 days, if loading is prevented. Exogenous GDFs stimulate tendon healing. This response is influenced by loading: without loading, cartilage and bone formation is initiated. This implies BMP signaling is crucial during tendon healing and influenced by mechanical loading. We therefore asked if mechanical loading influences the gene expression of the BMP signaling system in intact and healing tendons, and how the BMP signaling system changes during healing. The genes were four BMPs (OP-1/BMP-7, GDF-5/CDMP-1/BMP-14, GDF-6/CDMP-2/BMP-13, and GDF-7/CDMP-3/BMP-12), two receptors (BMPR1b and BMPR2), and the antagonists follistatin and noggin. The Achilles tendon was transected in rats and left to heal. Half of the rats had one Achilles tendon unloaded by injection of Botox in the calf muscles. Ten tendons were analyzed before transection and for each of four time points. All genes except noggin were expressed at all time points, but followed different patterns during healing. Loading strongly decreased the expression of follistatin, which could lead to increased signaling. The BMP system appears involved in tendon maintenance and healing, and may respond to mechanical loading.

译文

大鼠跟腱的愈合对机械负荷敏感,如果防止负荷,则在14天后愈伤组织强度降低3/4。外源性GDFs刺激肌腱愈合。这种反应受负荷影响: 在没有负荷的情况下,开始软骨和骨形成。这意味着BMP信号在肌腱愈合过程中至关重要,并受到机械负荷的影响。因此,我们询问机械负荷是否会影响完整和愈合肌腱中BMP信号系统的基因表达,以及BMP信号系统在愈合过程中如何变化。基因是四个bmp (OP-1/BMP-7,GDF-5/CDMP-1/BMP-14,GDF-6/CDMP-2/BMP-13和GDF-7/CDMP-3/BMP-12),两个受体 (BMPR1b和BMPR2) 以及拮抗剂follistatin和noggin。在大鼠中横切跟腱并使其愈合。通过在小腿肌肉中注射肉毒杆菌毒素,一半的大鼠的跟腱被卸载。在横切之前和四个时间点中的每一个都分析了十根肌腱。除noggin外,所有基因均在所有时间点表达,但在愈合过程中遵循不同的模式。负载强烈降低了卵泡抑素的表达,这可能导致信号传导增加。BMP系统似乎参与肌腱的维护和愈合,并且可能对机械负荷做出反应。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录