A test procedure for the determination of (2-methoxyethoxy)acetic acid (MEAA) was adapted and applied to urine samples from jet fuel (JP-8)-exposed mice using capillary gas chromatography with a mass selective detector (MSD). MEAA is a metabolite and proposed biomarker for exposure to 2-(2-methoxyethoxy)ethanol, a glycol ether component in the formulation of JP-8. The collected urine samples were spiked with deuterated butoxyacetic acid internal standard, and extracted with ethyl acetate, and esterified with ethanol and sulfuric acid, and the esters of the glycol ethers were extracted with methylene chloride. The chromatographic conditions used easily separate the MEAA ethyl ester from interferences within mouse urine. The application of this procedure to urine samples collected from mice demonstrated that MEAA was detectable after oral (2000 mg/kg) or dermal (50 mu L) exposure for 7 days to JP-8 at levels as high as 8.5 or 6.5 mu g/mL, respectively. This pilot demonstration indicated that total urinary MEAA was a viable biomarker for the two routes of JP-8 exposure in laboratory mice.

译文

采用了一种测定 (2-甲氧基乙氧基) 乙酸 (MEAA) 的测试程序,并使用带有质量选择检测器 (MSD) 的毛细管气相色谱法将其应用于暴露于喷气燃料 (JP-8) 的小鼠的尿液样品。MEAA是一种代谢物,并提出了暴露于2-(2-甲氧基乙氧基) 的生物标志物) 乙醇,JP-8制剂中的一种乙二醇醚成分。收集的尿样用氘代丁氧乙酸内标加标,用乙酸乙酯萃取,用乙醇和硫酸酯化,用二氯甲烷提取乙二醇醚的酯。使用的色谱条件很容易将MEAA乙酯与小鼠尿液中的干扰物分离。将该程序应用于从小鼠收集的尿液样本表明,MEAA在口服 (2000 mg/kg) 或真皮 (50μl) 后可检测到以高达8.5或6.5 μ g/mL的水平暴露于JP-8 7天,分别。该试验表明,总尿MEAA是实验室小鼠中JP-8暴露的两种途径的可行生物标志物。

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