The effects of prostaglandin E1 on cell-mediated cytotoxicity against hepatocytes were investigated using an in vitro cytotoxic assay system. Isolated liver cells from normal C57BL/6 mice were used as the target cells, and effector cells were obtained from spleens of C57BL/6 mice in which experimental hepatitis had been induced by immunization with syngeneic liver antigens. In this assay system, spleen T cells adhering to nylon wool demonstrated a high cytotoxic activity against target liver cells. The cytotoxicity was markedly reduced by prostaglandin E1 at concentrations greater than 10(-7) M. Maximum suppressive activity was obtained when prostaglandin E1 was continuously present during the assay period. By contrast, indomethacin, a specific inhibitor of prostaglandin synthesis, enhanced the cytotoxic activity of effector cells. These data seem to indicate that exogenously added prostaglandin E1 has an inhibitory effect on cell-mediated cytotoxicity of effector spleen cells against target hepatocytes.

译文

使用体外细胞毒性测定系统研究了前列腺素E1对细胞介导的对肝细胞的细胞毒性的影响。从正常C57BL/6小鼠分离的肝细胞用作靶细胞,并从C57BL/6小鼠的脾脏中获得效应细胞,其中通过用同基因肝抗原预防接种诱导了实验性肝炎。在此测定系统中,粘附在尼龙羊毛上的脾T细胞显示出对目标肝细胞的高细胞毒性活性。浓度大于10(-7) M的前列腺素E1显着降低了细胞毒性。当在测定期间连续存在前列腺素E1时,可获得最大的抑制活性。相比之下,吲哚美辛是前列腺素合成的特异性抑制剂,可增强效应细胞的细胞毒性活性。这些数据似乎表明,外源添加的前列腺素E1对效应脾细胞对靶肝细胞的细胞介导的细胞毒性具有抑制作用。

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