We examined seizure-susceptibility in a Drosophila model of human epilepsy using optogenetic stimulation of ReaChR (red-activatable channelrhodopsin). Photostimulation of the seizure-sensitive mutant parabss1 causes behavioral paralysis that resembles paralysis caused by mechanical stimulation, in many aspects. Electrophysiology shows that photostimulation evokes abnormal seizure-like neuronal firing in parabss1 followed by a quiescent period resembling synaptic failure and apparently responsible for paralysis. The pattern of neuronal activity concludes with seizure-like activity just prior to recovery. We tentatively identify the mushroom body as one apparent locus of optogenetic seizure initiation. The α/β lobes may be primarily responsible for mushroom body seizure induction.

译文

我们使用ReaChR (红色激活的通道视紫红质) 的光遗传学刺激检查了果蝇癫痫模型中的癫痫发作敏感性。在许多方面,对癫痫敏感的突变体parabss1的光刺激会导致行为麻痹,类似于机械刺激引起的麻痹。电生理学表明,光刺激会在parabss1中引起异常的癫痫样神经元放电,随后是类似于突触衰竭的静止期,显然是造成瘫痪的原因。神经元活动的模式在恢复之前以癫痫样活动结束。我们初步确定蘑菇体是光遗传发作开始的一个明显基因座。Α/β 裂片可能是引起蘑菇体癫痫发作的主要原因。

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