We have previously demonstrated that dark materials (DM) in acidified molasses are effectively adsorbed to Amberlite XAD7HP resin and are eluted from the resin with 0.1 M sodium hydroxide. In this paper, we have characterized the self-assembly behavior of molasses DM by using dynamic and static light scattering in combination with isoelectric focusing and infrared absorption spectroscopy in order to better understand the resin adsorption mechanism. One of DM derivatives, X-G2, contained carboxyl and hydroxyl groups and had a weight-average molar mass of 9.39 × 10(3) to 4.42 × 10(4) at pH 2.1-11.5. The aggregates retained their spherical shape over the full pH range and the large gyration radius (66.4-80.0 nm) indicated that the inner structure was loosely packed. Furthermore, X-G2 had an isoelectric point of 1.8, and its density increased sharply at pH 5.9 and then approached a nearly constant value under alkaline conditions. In summary, the self-assembly processes of DM are controlled by intermolecular hydrogen-bonding and hydrophobic interactions. The aggregates adsorb to the resin through hydrophobic interactions and are eluted when excess carboxylate anions are generated.

译文

我们先前已经证明,酸化糖蜜中的深色物质 (DM) 有效地吸附在Amberlite XAD7HP树脂上,并用0.1 M氢氧化钠从树脂中洗脱。在本文中,我们通过使用动态和静态光散射结合等电聚焦和红外吸收光谱来表征糖蜜DM的自组装行为,以便更好地了解树脂的吸附机理。X-G2,DM衍生物之一含有羧基和羟基,在pH 2.1-11.5下的重均摩尔质量为9.39 × 10(3) 至4.42 × 10(4)。聚集体在整个pH范围内保持其球形形状,并且大的回转半径 (66.4-80.0 nm) 表明内部结构松散堆积。此外,X-G2的等电点为1.8,其密度在pH 5.9时急剧增加,然后在碱性条件下接近几乎恒定的值。总之,DM的自组装过程受分子间氢键和疏水相互作用控制。聚集体通过疏水相互作用吸附到树脂上,并在产生过量的羧酸根阴离子时洗脱。

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