Polychromatic flow-cytometric assays were used to analyze paired intrahepatic and peripheral lymphocyte samples from 37 patients with chronic hepatitis C. Compared with peripheral cells, intrahepatic T cells were selectively enriched with CD45RO+ memory T cells but had a lower percentage of CD4+ T cells expressing the differentiation markers CD27 and CD28. The percentage of intrahepatic CD45RO+ and CD28+ T cells correlated with the degree of liver inflammation, which suggests that memory T cells at relatively early stages of differentiation are directly involved in liver inflammation. Despite their memory phenotype, intrahepatic T cells were defective in proliferation capability, produced less interferon- gamma in response to stimulation by T cell receptor, and contained less perforin but expressed higher levels of Fas and Fas ligand, compared with their counterparts in peripheral blood. The distinct characteristics of intrahepatic T cells suggest that they play an important role in the immunopathogenesis of chronic hepatitis C.

译文

使用多色流式细胞仪分析来自37例慢性丙型肝炎患者的成对的肝内和外周血淋巴细胞样本。与外周细胞相比,肝内T细胞选择性富集CD45RO记忆T细胞,但表达分化标记CD27和cd28的CD4 T细胞百分比较低。肝内CD45RO和CD28 T细胞的百分比与肝脏炎症程度相关,这表明处于分化相对早期的记忆T细胞直接参与了肝脏炎症。尽管肝内T细胞具有记忆表型,但其增殖能力存在缺陷,对T细胞受体的刺激产生的干扰素 γ 较少,并且穿孔素较少,但表达的Fas和Fas配体水平较高。血液。肝内T细胞的独特特征表明它们在慢性丙型肝炎的免疫发病中起重要作用。

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