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Salmonella enterica serotypes Derby, Mbandaka, Montevideo, Livingstone, and Senftenberg were among the 10 most prevalent serotypes isolated from farm animals in England and Wales in 1999. These serotypes are of potential zoonotic relevance; however, there is currently no "gold standard" fingerprinting method for them. A collection of isolates representing the former serotypes and serotype Gold Coast were analyzed using plasmid profiling, pulsed-field gel electrophoresis (PFGE), and ribotyping. The success of the molecular methods in identifying DNA polymorphisms was different for each serotype. Plasmid profiling was particularly useful for serotype Derby isolates, and it also provided a good level of discrimination for serotype Senftenberg. For most serotypes, we observed a number of nontypeable plasmid-free strains, which represents a limitation of this technique. Fingerprinting of genomic DNA by ribotyping and PFGE produced a significant variation in results, depending on the serotype of the strain. Both PstI/SphI ribotyping and XbaI-PFGE provided a similar degree of strain differentiation for serotype Derby and serotype Senftenberg, only marginally lower than that achieved by plasmid profiling. Ribotyping was less sensitive than PFGE when applied to serotype Mbandaka or serotype Montevideo. Serotype Gold Coast isolates were found to be nontypeable by XbaI-PFGE, and a significant proportion of them were found to be plasmid free. A similar situation applies to a number of serotype Livingstone isolates which were nontypeable by plasmid profiling and/or PFGE. In summary, the serotype of the isolates has a considerable influence in deciding the best typing strategy; a single method cannot be relied upon for discriminating between strains, and a combination of typing methods allows further discrimination.

译文

肠道沙门氏菌血清型Derby,Mbandaka,Montevideo,Livingstone和Senftenberg是1999年从英格兰和威尔士的农场动物中分离出的10种最普遍的血清型。这些血清型具有潜在的人畜共患病相关性; 但是,目前尚无针对它们的 “金标准” 指纹识别方法。使用质粒分析,脉冲场凝胶电泳 (PFGE) 和核糖分型分析了代表前血清型和血清型黄金海岸的分离株。每种血清型的分子方法在鉴定DNA多态性方面的成功是不同的。质粒分析对于血清型Derby分离株特别有用,并且还为血清型Senftenberg提供了良好的区分水平。对于大多数血清型,我们观察到许多不可分型的无质粒菌株,这代表了该技术的局限性。根据菌株的血清型,通过核糖分型和PFGE对基因组DNA进行指纹识别会产生显着的结果差异。PstI/SphI核型和XbaI-PFGE都为血清型Derby和血清型Senftenberg提供了相似的菌株分化程度,仅略低于通过质粒分析获得的菌株分化程度。当应用于血清型Mbandaka或血清型Montevideo时,核型分型不如PFGE敏感。通过XbaI-PFGE发现血清型黄金海岸分离株不可分型,并且发现其中很大一部分不含质粒。类似的情况适用于许多血清型利文斯通分离株,这些菌株无法通过质粒分析和/或PFGE进行分型。总而言之,分离株的血清型在决定最佳分型策略方面具有相当大的影响; 不能依靠单一方法来区分菌株,并且结合分型方法可以进一步区分。

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