In the past few years, new technologies have arisen that enable higher efficiency of gene editing. With the increase ease of using gene editing technologies, it is important to consider the best method for transferring new genetic material to livestock animals. Microinjection is a technique that has proven to be effective in mice but is less efficient in large livestock animals. Over the years, a variety of methods have been used for cloning as well as gene transfer including; nuclear transfer, sperm mediated gene transfer (SMGT), and liposome-mediated DNA transfer. This review looks at the different success rate of these methods and how they have evolved to become more efficient. As well as gene editing technologies, including Zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the most recent clustered regulatory interspaced short palindromic repeats (CRISPRs). Through the advancements in gene-editing technologies, generating transgenic animals is now more accessible and affordable. The goals of producing transgenic animals are to 1) increase our understanding of biology and biomedical science; 2) increase our ability to produce more efficient animals; and 3) produce disease resistant animals. ZFNs, TALENs, and CRISPRs combined with gene transfer methods increase the possibility of achieving these goals.

译文

在过去的几年中,出现了新技术,可以提高基因编辑的效率。随着基因编辑技术的使用越来越容易,重要的是要考虑将新的遗传物质转移到牲畜的最佳方法。微量注射是一种技术,已被证明对小鼠有效,但对大型家畜动物效率较低。多年来,已使用多种方法进行克隆和基因转移,包括: 核转移,精子介导的基因转移 (SMGT) 和脂质体介导的DNA转移。这篇评论着眼于这些方法的不同成功率,以及它们是如何演变成更有效率的。以及基因编辑技术,包括锌指核酸酶 (ZFNs),转录激活因子样效应核酸酶 (TALENs) 和最新的簇调节间隔短回文重复序列 (CRISPRs)。通过基因编辑技术的进步,现在更容易获得和负担得起转基因动物。生产转基因动物的目标是: 1) 增进我们对生物学和生物医学科学的了解; 2) 提高我们生产更高效动物的能力; 3) 生产抗病动物。ZFNs,TALENs和CRISPRs结合基因转移方法增加了实现这些目标的可能性。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录