A novel murine gene, designated ayk1, which encodes a putative serine/threonine kinase has been cloned and characterized. The predicted catalytic domain of the protein is highly similar to that of Drosophila aurora (62.9% identity), and to that of Saccharomyces Ipl1 (49.4% identity). All three proteins also have very basic calculated isoelectric points (higher than 10). aurora has been recently shown to be crucial for centrosome separation and chromosome segregation, while Ipl1 is essential for yeast viability and accurate chromosome segregation. The results of Northern analysis and in situ RNA localization support a similar role for ayk1. The gene is specifically expressed in meiotically active cells, and during spermatogenesis, ayk1 transcripts accumulate just before the first meiotic division. Much lower levels are found in mitotically active cells. We propose that Ayk1, aurora and Ipl1 belong to a distinct new subfamily of kinases. These results suggest that the pathways controlling chromosome segregation are evolutionary conserved, and that similar control mechanisms operate in mitosis and meiosis.

译文

已克隆并鉴定了一种新的鼠基因ayk1,该基因编码假定的丝氨酸/苏氨酸激酶。该蛋白质的预测催化结构域与果蝇aurora的催化结构域 (62.9% 身份) 和酵母ipp1的催化结构域 (49.4% 身份) 高度相似。这三种蛋白质也都有非常基本的计算等电点 (高于10)。aurora最近被证明对于中心体分离和染色体分离至关重要,而Ipl1对于酵母活力和准确的染色体分离至关重要。Northern分析和原位RNA定位的结果支持ayk1的类似作用。该基因在减数活性细胞中特异性表达,在精子发生过程中,ayk1转录本在第一次减数分裂分裂之前就积累了。在有丝分裂活性的细胞中发现的水平要低得多。我们建议Ayk1,aurora和ipp1属于一个独特的新激酶亚科。这些结果表明,控制染色体分离的途径是进化保守的,并且类似的控制机制在有丝分裂和减数分裂中起作用。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录