Adenine monophosphate (AMP) activated protein kinase (AMPK) is an important regulator of obesity. The objective of the present work was to study and compare AMPK protein expression in visceral vs. subcutaneous adipose tissue of morbid obese subjects and to correlate it with adipose tissue characteristics. We selected a total population of 17 extreme obese (BMI>or=40 kg/m2) aged 42.8+/-10.2 years were included in this study. We measured anthropometric and body composition parameters. Adiponectin expression by qRT-PCR, isoproterenol-stimulated lipolytic rates, and AMPK alpha subunits expression by Western blot in adipose tissue explants were determined. Finally plasma concentrations of glucose, triacylglycerols, total cholesterol, HDL-c, LDL-c and insulin were also measured. Our results showed that AMPK alpha expression was higher in subcutaneous than in visceral tissue. A positive correlation between AMPK expression and adiponectin expression in human subcutaneous adipose tissue was observed. Furthermore, a positive correlation between AMPK expression and isoproterenol evoked upregulation of lipolysis rate was also observed. In conclusion, AMPK alpha expression differed according to adipose tissue location. The positive correlation between subcutaneous adipose tissue AMPK and adiponectin or the evoked lipolysis rate could indicate a protective role of AMPK in this tissue, counteracting insulin resistance in morbid obese patients.

译文

腺嘌呤单磷酸 (AMP) 活化蛋白激酶 (AMPK) 是肥胖的重要调节因子。本工作的目的是研究和比较病态肥胖受试者的内脏和皮下脂肪组织中AMPK蛋白的表达,并将其与脂肪组织特征相关联。我们选择了年龄在42.8/-10.2岁的17个极端肥胖 (BMI> 或 = 40千克/m2) 的总人口。我们测量了人体测量和身体成分参数。测定了脂肪组织外植体中qRT-PCR的脂联素表达,异丙肾上腺素刺激的脂解速率和蛋白质印迹的AMPK α 亚基表达。最后,还测量了葡萄糖,三酰甘油,总胆固醇,hdl-c,ldl-c和胰岛素的血浆浓度。我们的结果表明,皮下组织中的AMPK α 表达高于内脏组织。观察到人皮下脂肪组织中AMPK表达与脂联素表达呈正相关。此外,还观察到AMPK表达与异丙肾上腺素引起的脂解速率上调之间呈正相关。总之,AMPK α 的表达根据脂肪组织的位置而有所不同。皮下脂肪组织AMPK和脂联素之间的正相关或诱发的脂解率可能表明AMPK在该组织中的保护作用,抵消病态肥胖患者的胰岛素抵抗。

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