Cationic liposomes can efficiently carry nucleic acids into mammalian cells. This property is tightly connected with their ability to fuse with negatively charged natural membranes (i.e. the plasma membrane and endosomal membrane). We used FRET to monitor and compare the efficiency of lipid mixing of two liposomal preparations--one of short-chained diC14-amidine and one of long-chained unsaturated DOTAP--with the plasma membrane of HeLa cells. The diC14-amidine liposomes displayed a much higher susceptibility to lipid mixing with the target membranes. They disrupted the membrane integrity of the HeLa cells, as detected using the propidium iodide permeabilization test. Morphological changes were transient and essentially did not affect the viability of the HeLa cells. The diC14-amidine liposomes were much more effective at either inducing lipid mixing or facilitating transfection.

译文

:阳离子脂质体可以有效地将核酸带入哺乳动物细胞。这种特性与其与带负电的天然膜(即质膜和内体膜)融合的能力紧密相关。我们使用FRET监测和比较两种脂质体制剂(一种短链diC14--和一种长链不饱和DOTAP)与HeLa细胞质膜的脂质混合效率。 diC14-am脂质体对脂质与靶膜混合表现出更高的敏感性。使用碘化丙啶通透性测试检测到,它们破坏了HeLa细胞的膜完整性。形态变化是短暂的,基本上不影响HeLa细胞的生存能力。 diC14-am脂质体在诱导脂质混合或促进转染方面更为有效。

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