BACKGROUND:Wound healing is a dynamic process, involving the recruitment of growth factors, cytokines, chemokines and cellular populations. Recently, the Cord Blood Platelet Gel (CBPG) has been applied successfully in wound closure and tissue regeneration. Moreover, its proper combination with stem cell populations such as Mesenchymal Stromal Cells (MSCs) may positively improve the wound healing process. Based on the above data, this study aimed to the evaluation of wound healing capacity of MSCs combined with CBPG under in vitro conditions. METHODS:Initially, CBPG was developed from Cord Blood Units (CBUs). The determination of wound healing ability of MSCs was performed using the scratch wound assay. In addition, the morphological features, immunophenotypical characteristics and differentiation capacity of MSCs were evaluated. RESULTS:Scratch wound assay results showed, that CBPG could positively stimulate the MSCs migration. Moreover, MSCs cultured in presence of CBPG were characterized by elongated shape and improved stemness properties as it was indicated by flow cytometric analysis and differentiation process. CONCLUSION:These results clearly showed the beneficial effect of CBPG in combination with MSCs in wound healing. The proper combination of CBPG with stem cells strategy may enhance the healing process in patients with skin erosions.

译文

背景:伤口愈合是一个动态过程,涉及生长因子,细胞因子,趋化因子和细胞群的募集。最近,脐带血血小板凝胶(CBPG)已成功应用于伤口闭合和组织再生。此外,将其与干细胞群体(如间质基质细胞(MSC))适当组合可积极改善伤口愈合过程。基于上述数据,本研究旨在评估在体外条件下结合CBPG的MSCs的伤口愈合能力。
方法:最初,CBPG是从脐带血单位(CBU)开发的。 MSCs的伤口愈合能力的测定是使用刮擦伤口测定法进行的。此外,评价了MSC的形态特征,免疫表型特征和分化能力。
结果:刮伤试验结果表明,CBPG可以积极刺激MSC的迁移。而且,通过流式细胞术分析和分化过程表明,在CBPG存在下培养的MSC具有细长的形状和改善的干特性。
结论:这些结果清楚地表明,CBPG与MSC联合在伤口愈合中具有有益作用。 CBPG与干细胞策略的适当组合可增强皮肤糜烂患者的愈合过程。

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