BACKGROUND:Oral squamous cell carcinoma (OSCC) is a life-threatening disease. It could be preceded by oral potentially malignant disorders (OPMDs). It was confirmed that chronic inflammation can promote carcinogenesis. Cytokines play a crucial role in this process. The aim of the study was to evaluate interleukin-1alpha (IL-1α), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor alpha (TNF-α) in tissue specimens and saliva of patients with OSCC and OPMDs. METHODS:Cytokines were evaluated in 60 tissue specimens of pathological lesions (OSCCs or OPMDs) and in 7 controls (normal oral mucosa, NOM) by immunohistochemistry and in saliva of 45 patients with OSCC or OPMDs and 9 controls (healthy volunteers) by enzyme-linked immunosorbent assays. RESULTS:Immunohistochemical analysis revealed significantly higher expression of IL-8 in OSCC specimens and TNF-α in OSCCs and OPMDs with dysplasia as compared to NOM. Moreover, expression of TNF-α was significantly higher in oral leukoplakia and oral lichen planus without dysplasia, whereas expression of IL-8 only in oral leukoplakia without dysplasia in comparison with NOM. Salivary concentrations of all evaluated cytokines were significantly higher in patients with OSCC than in controls. Moreover, levels of IL-8 were significantly higher in saliva of patients with OPMDs with dysplasia as compared to controls and in OSCC patients as compared to patients with dysplastic lesions. There was also significant increase in salivary concentrations of IL-6, IL-8 and TNF-α in patients with OSCC as compared to patients with OPMDs without dysplasia. CONCLUSION:The study confirmed that proinflammatory, NF-kappaB dependent cytokines are involved in pathogenesis of OPMDs and OSCC. The most important biomarker of malignant transformation process within oral mucosa among all assessed cytokines seems to be IL-8. Further studies on a larger sample size are needed to corroborate these results.

译文

背景:口腔鳞状细胞癌(OSCC)是一种威胁生命的疾病。在此之前可能会出现口腔潜在的恶性疾病(OPMD)。证实了慢性炎症可以促进癌变。细胞因子在这一过程中起着至关重要的作用。该研究的目的是评估组织标本和唾液中的白细胞介素-1α(IL-1α),白细胞介素6(IL-6),白细胞介素8(IL-8)和肿瘤坏死因子α(TNF-α)。 OSCC和OPMDs患者的比例。
方法:采用免疫组织化学方法对60例病理性病变(OSCCs或OPMDs)组织标本和7例对照(正常口腔粘膜,NOM)的细胞因子进行了评估,对45例OSCC或OPMDs患者的唾液中的细胞因子进行了评估,对9例对照(健康志愿者)进行了酶促评估链接的免疫吸附测定。
结果:免疫组织化学分析显示,不典型增生的OSCC和OPMD中IL-8在OSCC标本中的表达显着升高,TNF-α的表达明显高于NOM。此外,与NOM相比,在没有发育异常的口腔白斑和口腔扁平苔藓中,TNF-α的表达显着较高,而在没有发育异常的口腔白斑中IL-8的表达只有NOM。 OSCC患者的所有评估细胞因子的唾液浓度均显着高于对照组。此外,与对照组相比,发育异常的OPMD患者唾液中的IL-8水平显着高于对照组,而在OSCC患者中与发育异常病变的患者相比,IL-8水平更高。与没有发育异常的OPMD患者相比,OSCC患者唾液中IL-6,IL-8和TNF-α的浓度也显着增加。
结论:该研究证实促炎性,NF-κB依赖性细胞因子与OPMDs和OSCC的发病有关。在所有评估的细胞因子中,口腔黏膜内恶性转化过程最重要的生物标志物似乎是IL-8。为了证实这些结果,需要对更大的样本量进行进一步的研究。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录