BACKGROUND:Interleukin 1 (IL-1) is involved in bone resorption. However, the role of IL-1 in periapical lesions characterized by periapical bone destruction in primary teeth has not yet been fully elucidated. This study aimed to detect the distribution and expression of IL-1 in periapical lesions in primary teeth and assess the relationship between the cytokines and the degree of inflammatory cell infiltration.
METHODS:A total of 106 chronic periapical lesions in primary teeth were harvested. Haematoxylin and eosin (H&E) staining was used to determine the histological type and the inflammatory cell infiltration grade (mild, moderate, and severe), and immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were used to detect the distribution and expression of IL-1α and IL-1β.
RESULTS:Of the 106 chronic periapical lesion samples, there were 85 cases of periapical granuloma, accounting for 80.19% of the total samples, and 21 cases of radicular cysts, accounting for 19.81%; no cases of abscess were detected. Immunohistochemistry results showed that both IL-1α and IL-1β were expressed in periapical granulomas and cysts. ELISA results showed that IL-1α and IL-1β levels were higher in the periapical granuloma group than in the radicular cyst and normal control groups (P < 0.05). In the periapical granuloma group, IL-1α and IL-1β were detected at higher levels in the severe inflammatory cell infiltration subgroup than in the mild-inflammatory cell infiltration subgroup (P < 0.05), and IL-1β expression was also higher in the moderate inflammatory cell infiltration subgroup than in the mild inflammatory cell infiltration subgroup (P < 0.01). A significant positive correlation was observed between the protein expression levels of IL-1α and IL-1β and the inflammation grade in periapical granulomas from primary teeth (P < 0.05).
CONCLUSION:Expression levels of the cytokines IL-1α and IL-1β in periapical granulomas from primary teeth increased with increasing inflammatory severity and appeared to be a contributing factor to the progression of periapical lesions.
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【Increased interleukin 1α and interleukin 1β expression is involved in the progression of periapical lesions in primary teeth.].
【白细胞介素1α和白介素1β表达的增加与乳牙根尖周病变的发展有关。】
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DOI：10.1186/s12903-018-0586-3文章类型：杂志文章
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背景：白介素1（IL-1）参与骨吸收。然而，IL-1在以乳牙根尖周围骨破坏为特征的根尖周围病变中的作用尚未完全阐明。这项研究旨在检测乳牙根尖周病变中IL-1的分布和表达，并评估细胞因子与炎性细胞浸润程度之间的关系。
方法：总共收集了106例乳牙的慢性根尖周病变。使用苏木精和曙红（H＆E）染色确定组织学类型和炎性细胞浸润等级（轻度，中度和重度），并使用免疫组化和酶联免疫吸附法（ELISA）检测IL的分布和表达-1α和IL-1β。
结果：106例慢性根尖周病变标本中，根尖肉芽肿85例，占总标本的80.19％；根尖囊肿21例，占19.81％。没有发现脓肿病例。免疫组织化学结果显示，IL-1α和IL-1β均在根尖肉芽肿和囊肿中表达。 ELISA结果显示，根尖肉芽肿组的IL-1α和IL-1β水平高于根尖囊肿和正常对照组（P <0.05）。在根尖肉芽肿组中，重度炎性细胞浸润亚组中IL-1α和IL-1β的水平高于轻度炎性细胞浸润亚组（P <0.05），IL-1β的表达也高于轻度炎性细胞浸润组。中度炎症细胞浸润亚组比轻度炎症细胞浸润亚组（P <0.01）。乳齿根尖肉芽肿中IL-1α和IL-1β的蛋白表达水平与炎症程度呈显着正相关（P <0.05）。
结论：乳牙根尖肉芽肿中细胞因子IL-1α和IL-1β的表达水平随着炎症程度的增加而增加，并可能是导致根尖周病变发展的因素。
方法：总共收集了106例乳牙的慢性根尖周病变。使用苏木精和曙红（H＆E）染色确定组织学类型和炎性细胞浸润等级（轻度，中度和重度），并使用免疫组化和酶联免疫吸附法（ELISA）检测IL的分布和表达-1α和IL-1β。
结果：106例慢性根尖周病变标本中，根尖肉芽肿85例，占总标本的80.19％；根尖囊肿21例，占19.81％。没有发现脓肿病例。免疫组织化学结果显示，IL-1α和IL-1β均在根尖肉芽肿和囊肿中表达。 ELISA结果显示，根尖肉芽肿组的IL-1α和IL-1β水平高于根尖囊肿和正常对照组（P <0.05）。在根尖肉芽肿组中，重度炎性细胞浸润亚组中IL-1α和IL-1β的水平高于轻度炎性细胞浸润亚组（P <0.05），IL-1β的表达也高于轻度炎性细胞浸润组。中度炎症细胞浸润亚组比轻度炎症细胞浸润亚组（P <0.01）。乳齿根尖肉芽肿中IL-1α和IL-1β的蛋白表达水平与炎症程度呈显着正相关（P <0.05）。
结论：乳牙根尖肉芽肿中细胞因子IL-1α和IL-1β的表达水平随着炎症程度的增加而增加，并可能是导致根尖周病变发展的因素。
