Research models of infarction and myocardial ischemia are essential to investigate the acute and chronic pathobiological and pathophysiological processes in myocardial ischemia and to develop and optimize future treatment. Two different methods of creating myocardial ischemia are performed in laboratory rodents. The first method is to create cryo infarction, a fast but inaccurate technique, where a cryo-pen is applied on the surface of the heart (1-3). Using this method the scientist can not guarantee that the cryo-scar leads to ischemia, also a vast myocardial injury is created that shows pathophysiological side effects that are not related to myocardial infarction. The second method is the permanent ligation of the left anterior descending artery (LAD). Here the LAD is ligated with one single stitch, forming an ischemia that can be seen almost immediately. By closing the LAD, no further blood flow is permitted in that area, while the surrounding myocardial tissue is nearly not affected. This surgical procedure imitates the pathobiological and pathophysiological aspects occurring in infarction-related myocardial ischemia. The method introduced in this video demonstrates the surgical procedure of a mouse infarction model by ligating the LAD. This model is convenient for pathobiological and pathophysiological as well as immunobiological studies on cardiac infarction. The shown technique provides high accuracy and correlates well with histological sections.

译文

:梗塞和心肌缺血的研究模型对于调查心肌缺血的急,慢性病理生物学和病理生理过程以及制定和优化未来治疗至关重要。在实验室啮齿动物中进行了两种不同的产生心肌缺血的方法。第一种方法是制造冰冻梗塞,这是一种快速但不准确的技术,其中在心脏表面上使用冰冻笔(1-3)。使用这种方法,科学家无法保证低温疤痕会导致局部缺血,还会造成巨大的心肌损伤,这种损伤显示出与心肌梗塞无关的病理生理学副作用。第二种方法是永久结扎左前降支动脉(LAD)。在这里,LAD结扎了一个针脚,形成了几乎可以立即看到的局部缺血。通过关闭LAD,该区域将不允许进一步的血液流动,而周围的心肌组织几乎不会受到影响。该手术过程模仿了与梗塞相关的心肌缺血中发生的病理生物学和病理生理学方面。该视频中介绍的方法通过结扎LAD演示了小鼠梗塞模型的外科手术过程。该模型对于心脏梗死的病理生物学和病理生理以及免疫生物学研究是方便的。所示技术提供了很高的准确性,并与组织学切片很好地相关。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录