This study investigated the effect of a high-fat (HF) diet on protein expression of leptin and its receptor in the gonads of dams and their offspring. Female Wistar rats were fed a HF diet (30% fat) or a standard breeding (BD) diet (5% fat) during pregnancy and lactation. At 21 days of lactation, mothers and both sexes of prepubertal offspring were killed by decapitation. The protein expression of leptin and its receptor was assayed by Western blot and immunohistochemistry in gonadal, periovarian, and epididymal white adipose tissue (WAT). We demonstrated that leptin protein expression in ovary, and both leptin and ObRb expression in periovarian WAT was decreased in HF dams compared with BD animals. Immunohistochemistry showed lower leptin expression in growing antral follicles and corpora lutea of HF dams. Conversely, in both gonads and epididymal WAT of HF offspring, leptin and its receptor were significantly higher expressed compared with BD. Immunolocalization of leptin system in HF offspring gonads showed higher expression in growing and antral follicles of the ovary, seminiferous tubules, and interstitial tissue of testes. In conclusion, high gonadal and gonadal-WAT expression of leptin system was observed in the offspring of dams fed a HF diet during pregnancy and lactation.

译文

:这项研究调查了高脂饮食对大坝及其后代性腺中瘦素及其受体蛋白表达的影响。在怀孕和哺乳期间,给雌性Wistar大鼠喂食HF日粮(30%脂肪)或标准繁殖(BD)日粮(5%脂肪)。哺乳期21天,母亲和青春期前的后代均被斩首处决。用蛋白质印迹法和免疫组织化学法检测性腺,卵巢周和附睾白色脂肪组织(WAT)中瘦素及其受体的蛋白表达。我们证明,与BD动物相比,HF大坝中卵巢中的瘦素蛋白表达以及卵巢周WAT中的瘦素和ObRb表达均降低。免疫组织化学显示,HF大坝的肛门窦卵泡和黄体中瘦素的表达较低。相反,在HF后代的性腺和附睾WAT中,瘦素及其受体的表达均显着高于BD。瘦素系统在HF后代性腺中的免疫定位显示在卵巢,生精小管和睾丸间质组织的生长和窦房滤泡中更高的表达。总之,在妊娠和哺乳期喂食HF饮食的大坝的后代中,观察到瘦素系统的性腺和性腺WAT高表达。

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