A specific chorion peroxidase is present in Aedes aegypti and this enzyme is responsible for catalyzing chorion protein cross-linking through dityrosine formation during chorion hardening. Peroxidase-mediated dityrosine cross-linking requires H(2)O(2), and this study discusses the possible involvement of the chorion peroxidase in H(2)O(2) formation by mediating NADH/O(2) oxidoreduction during chorion hardening in A. aegypti eggs. Our data show that mosquito chorion peroxidase is able to catalyze pH-dependent NADH oxidation, which is enhanced in the presence of Mn(2+). Molecular oxygen is the electron acceptor during peroxidase-catalyzed NADH oxidation, and reduction of O(2) leads to the production of H(2)O(2), demonstrated by the formation of dityrosine in a NADH/peroxidase reaction mixture following addition of tyrosine. An oxidoreductase capable of catalyzing malate/NAD(+) oxidoreduction is also present in the egg chorion of A. aegypti. The cooperative roles of chorion malate/NAD(+)oxidoreductase and chorion peroxidase on generating H(2)O(2) with NAD(+) and malate as initial substrates were demonstrated by the production of dityrosine after addition of tyrosine to a reaction mixture containing NAD(+) and malate in the presence of both malate dehydrogenase fractions and purified chorion peroxidase. Data suggest that chorion peroxidase-mediated NADH/O(2) oxidoreduction may contribute to the formation of the H(2)O(2) required for chorion protein cross-linking mediated by the same peroxidase, and that the chorion associated malate dehydrogenase may be responsible for the supply of NADH for the H(2)O(2) production.

译文

埃及伊蚊中存在特定的绒毛膜过氧化物酶,该酶负责在绒毛膜硬化过程中通过二酪氨酸形成催化绒毛膜蛋白交联。过氧化物酶介导的二氢酪氨酸交联需要H(2)O(2),本研究讨论了绒毛膜过氧化物酶在H(2)O(2)形成中的可能参与,通过在绒毛膜硬化过程中介导NADH / O(2)氧化还原在埃及埃及鸡蛋中。我们的数据表明,蚊绒毛膜过氧化物酶能够催化pH依赖性的NADH氧化,在存在Mn(2)的情况下,氧化作用会增强。分子氧是过氧化物酶催化的NADH氧化过程中的电子受体,O(2)的还原导致H(2)O(2)的产生,这是通过在NADH /过氧化物酶的反应混合物中添加二氢酪氨酸形成的二氢酪氨酸所证实的。酪氨酸。埃及曲霉的卵绒毛膜中也存在能够催化苹果酸/ NAD()氧化还原的氧化还原酶。苹果酸/ NAD()氧化还原酶和绒毛膜过氧化物酶在以NAD()和苹果酸为起始底物生成H(2)O(2)时的协同作用通过向含有NAD的反应混合物中添加酪氨酸后生成二酪氨酸来证明()和苹果酸,同时存在苹果酸脱氢酶馏分和纯化的绒毛膜过氧化物酶。数据表明绒毛膜过氧化物酶介导的NADH / O(2)氧化还原可能有助于由相同的过氧化物酶介导的绒毛膜蛋白交联所需的H(2)O(2)的形成,并且绒毛膜相关的苹果酸脱氢酶可能负责为H(2)O(2)生产提供NADH。

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