The expression of GABA in the human fetal (12-25 weeks of gestation), postnatal (five-month-old), and adult (35-year-old) retinas was investigated by immunohistochemistry. GABA expression was seen as early as 12 weeks in the undifferentiated cells of the inner neuroblast zone; a few optic nerve fiber layer axons were clearly labeled, suggesting that some of the stained cell bodies were prospective ganglion cells, others could be displaced amacrine cells. From 16-17 to 24-25 weeks, intense labeling was found in the amacrine, displaced amacrine, and some ganglion cells. During this time period, horizontal cells (identified by calbindin immunohistochemistry), undergoing migration (periphery) and differentiation (center), expressed GABA prominently. In the postnatal retina, some horizontal cells were moderately labeled, but very weakly in a few cells, in the adult. The Müller cells developed immunoreactivity first weakly at 12 weeks and then moderately from 16-17 weeks onward. The staining was also evident in the postnatal and adult retinas, showing labeled processes of these glial cells. Virtually no axons in the adult optic nerve and nerve fiber layer were stained; the staining was restricted to a few, large ganglion cells and displaced amacrine cellsSome amacrines were also labeled. The possibility that GABA might play a role in horizontal cell differentiation and maturation is highlighted. Other evidences suggest that GABA might play a role in metabolism during retinal development.

译文

通过免疫组织化学研究了GABA在人胎儿(妊娠12-25周),产后(五个月大)和成年(35岁)视网膜中的表达。早在12周内神经母细胞区未分化的细胞中就可以看到GABA的表达。一些视神经纤维层轴突被清楚地标记,表明某些染色的细胞体是预期的神经节细胞,其他可能是置换的无长突细胞。从16-17周到24-25周,在无长蛋白,移位的无长蛋白和一些神经节细胞中发现了强烈的标记。在这段时间内,水平细胞(通过钙结合蛋白免疫组织化学鉴定),经历迁移(外围)和分化(中心),主要表达GABA。在成年后的视网膜中,成人中一些水平细胞被中等程度标记,但少数细胞中非常弱。 Müller细胞首先在12周时出现弱免疫反应,然后在16-17周后逐渐出现免疫反应。染色在产后和成年视网膜中也很明显,显示出这些神经胶质细胞的标记过程。成人视神经和神经纤维层几乎没有染色。染色仅限于少数大型神经节细胞和置换的无长突细胞。一些无长突也被标记。强调了GABA可能在水平细胞分化和成熟中发挥作用的可能性。其他证据表明,GABA可能在视网膜发育过程中的代谢中发挥作用。

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