Porcine epidemic diarrhea virus (PEDV) is an emerging and re-emerging viral pathogen of pigs that is causing significant economic concerns in the global pork industry. The virulent G2b PEDV subgroup, which was responsible for the 2013-2014 pandemics on the American and Asian continents, hit Jeju Island in 2014, which had been PEDV-free since 2004. This study presents molecular characterization of a novel PEDV variant with a 5-amino acid (aa) insertion (DTHPE) in the spike (S) gene (an S-insertion) that was identified in diarrheic piglets from Jeju Island. The genome of the PEDV strain KOR/KNU-1601/2016 was sequenced and analyzed to characterize the S-insertion variant circulating on Jeju Island. The full-length genome sequence of KNU-1601 is 28,053 nucleotides (nt) in length, which is 24 nt and 15 nt longer than the genome sequences of G1 classical and G2 epidemic field strains, respectively. KNU-1601 shares 99.5-99.6% nucleotide sequence identity at the genome level and 98.0-98.6% amino acid sequence identity at the S gene level with other global G2b PEDV strains. Genetic and phylogenetic analyses indicated that the KNU-1601 variant is most closely related to the G2b field isolates but appears to undergo continuous evolution in the field. These data advance our understanding of the genetic diversity and evolutionary characteristics of the PEDV field strains circulating in South Korea.

译文

:猪流行性腹泻病毒(PEDV)是一种新兴的且正在重新出现的猪病毒病原体,正在引起全球猪肉行业的重大经济担忧。有毒的G2b PEDV亚组是造成美洲和亚洲大陆2013-2014年大流行的原因,于2014年袭击了济州岛,该岛自2004年以来一直没有PEDV。 -氨基酸(aa)插入(DTHPE)到穗(S)基因(S插入)中,该基因在来自济州岛的腹泻仔猪中鉴定。对PEDV株KOR / KNU-1601 / 2016的基因组进行了测序和分析,以表征在济州岛上传播的S插入变异体。 KNU-1601的全长基因组序列长度为28,053个核苷酸(nt),分别比经典G1和G2流行病菌株的基因组序列长24 nt和15 nt。 KNU-1601与其他全球G2b PEDV菌株在基因组水平上具有99.5-99.6%的核苷酸序列同一性,在S基因水平上具有98.0-98.6%的氨基酸序列同一性。遗传和系统发育分析表明,KNU-1601变体与G2b田间分离株关系最密切,但似乎在田间经历了不断的进化。这些数据使我们对在韩国流通的PEDV野毒株的遗传多样性和进化特征有了更深入的了解。

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