Memory impairment is the most common symptom in patients with Alzheimer's disease (AD). Angelica keiskei (AK) has traditionally been used as a diuretic, laxative, analeptic and galactagogue. However, the anti-amnesic effects of AK and its molecular mechanisms have yet to be clearly elucidated. The aim of the present study is to evaluate the effects of AK on scopolamine-induced memory impairments in mice. The regulatory effect of AK on memory impairment was investigated using passive avoidance, Y-maze and the Morris water maze tasks. Acetylcholinesterase (AChE) activity assay was performed to investigate the cholinergic antagonistic effect of AK in the hippocampus. The effect of AK on phosphorylation of cAMP response element-binding protein (CREB) and expression of brain-derived neurotrophic factor (BDNF) were evaluated by Western blot assays and immunohistochemistry. The findings showed that AK significantly attenuated scopolamine-induced cognitive impairment in mice. Increase of AChE activity caused by scopolamine was significantly attenuated by AK. Additionally, AK significantly recovered the phosphorylation of CREB and expression of BDNF reduced by scopolamine in the hippocampus. Taken together, these results provide experimental evidence that AK might be a useful agent in preventing deficit of learning and memory caused by AD and aging.

译文

记忆力减退是阿尔茨海默氏病(AD)患者最常见的症状。当归(AK)传统上已被用作利尿剂,泻药,镇痛药和催乳剂。但是,AK的抗记忆效应及其分子机制尚未明确阐明。本研究的目的是评估AK对东pol碱诱导的小鼠记忆障碍的影响。使用被动回避,Y迷宫和Morris水迷宫任务研究了AK对记忆障碍的调节作用。进行乙酰胆碱酯酶(AChE)活性测定以研究AK对海马的胆碱能拮抗作用。通过蛋白质印迹法和免疫组织化学评估了AK对cAMP反应元件结合蛋白(CREB)磷酸化和脑源性神经营养因子(BDNF)表达的影响。研究结果表明,AK可以显着减轻东pol碱引起的小鼠认知障碍。东碱引起的AChE活性增加被AK明显减弱。此外,AK可显着恢复海马中东pol碱所致的CREB磷酸化和BDNF的表达。综上所述,这些结果提供了实验证据,表明AK可能是预防由AD和衰老引起的学习和记忆不足的有用药物。

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