Porcine enzootic pneumonia (PEP), which is caused by the fastidious bacterium Mycoplasma hyopneumoniae, is one of the most economically important diseases in the pig industry worldwide. Commercial bacterins provide only partial protection; therefore, the development of more efficient vaccines against PEP is necessary. In this study, the cellular and humoral immune responses elicited by DNA and recombinant subunit vaccines based on the P37, P42, P46 and P95 antigens of M. hyopneumoniae were evaluated after the intramuscular inoculation of BALB/c mice. The expression of the cytokines INFγ, TNFα and IL1 was evaluated by real-time RT-PCR in splenocytes from vaccinated mice. All antigens delivered as subunit vaccines, especially P42 and P95, and the pcDNA3/P46 DNA vaccine were able to elicit strong immune responses. These vaccines induced cellular immune responses and the production of antibodies able to react with native M. hyopneumoniae proteins. Because both cellular and humoral immune responses were induced, P42 and P95 are promising candidates for a recombinant subunit vaccine and P46 is a promising candidate for a DNA vaccine against PEP.

译文

猪传染性肺炎(PEP)是由细菌性猪肺炎支原体(Mycoplasma hyopneumoniae)引起的,是全球养猪业中最重要的经济疾病之一。商业细菌仅提供部分保护;因此,有必要开发出更有效的抗PEP疫苗。在这项研究中,肌肉注射BALB / c小鼠后,评估了基于猪肺炎支原体的P37,P42,P46和P95抗原的DNA和重组亚基疫苗引发的细胞和体液免疫反应。通过实时RT-PCR评估来自接种小鼠的脾细胞中细胞因子INFγ,TNFα和IL1的表达。所有作为亚单位疫苗提供的抗原,特别是P42和P95,以及pcDNA3 / P46 DNA疫苗都能够引起强烈的免疫反应。这些疫苗诱导细胞免疫反应,并产生能够与天然猪肺炎支原体蛋白反应的抗体。因为诱导了细胞和体液免疫应答,所以P42和P95是重组亚单位疫苗的有前途的候选者,而P46是抗PEP的DNA疫苗的有前途的候选者。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录