Four monoclonal antibodies reactive with the rod portion of human cytokeratin No. 8 were evaluated using experimental tumour radio-immunolocalization. Nude mice carrying human HeLa cell tumours were injected with 125I-labelled F(ab')2 fragments or intact IgG antibodies. Positive immunolocalization was observed with all the specific antibodies, the intact antibodies being superior to their analogous F(ab')2 fragments. High localization efficiency was observed despite the fact that the targets are intracellular filaments. The presence of necrotic areas in the tumours may favour the high uptake in the tumours. By computerized gamma-scintigraphic evaluation of the antibody distribution in the animals, several kinetic parameters of the antibody behaviour in vivo could be determined. The biological half-lives in the tumours were determined to be greater than 600 h for some of the antibodies compared to a half-life of 40-80 h in non-tumour tissues. Optimal imaging was obtained as late as 10-14 days after administration of the antibody. Up to 10% of the injected antibody could be identified in the tumours, indicating a potential value in radio-immunolocalization and radio-immunotherapy using anticytokeratin antibodies.

译文

:使用实验性肿瘤放射免疫定位,评估了与8号人角蛋白棒部分有反应性的四种单克隆抗体。给携带人HeLa细胞肿瘤的裸鼠注射125I标记的F(ab')2片段或完整的IgG抗体。在所有特异性抗体中均观察到阳性免疫定位,完整抗体优于其类似的F(ab')2片段。尽管目标是细胞内细丝,但仍观察到高的定位效率。肿瘤中坏死区域的存在可能有利于肿瘤的高摄取。通过计算机对动物体内抗体分布的伽马闪烁显像评估,可以确定体内抗体行为的几个动力学参数。对于某些抗体,肿瘤中的生物学半衰期确定为大于600小时,而在非肿瘤组织中,其半衰期为40-80小时。抗体给药后的10-14天后可获得最佳成像。可以在肿瘤中鉴定出多达10%的注射抗体,这表明在使用抗细胞角蛋白抗体进行放射免疫定位和放射免疫治疗中具有潜在价值。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录