High resolution 1H NMR spectroscopy of biofluids, cells and tissue extracts allows rapid, non destructive analysis for a wide range of metabolites and organic compounds with minimal sample pre treatment. We have applied high resolution 1H NMR spectroscopy to investigate the biochemical effects of Cu II in two earthworm species Eisenia andrei n =78 and Lumbricus rubellus n =45 exposed under laboratory and semi field conditions respectively. The most marked metabolic response was the elevation of endogenous whole body free histidine in animals which positively correlated with increasing copper exposure and total copper burden in the semi field experiment. Histidine forms thermodynamically stable copper complexes under a wide range of physico chemical conditions and we proposed that the elevation of free histidine in response to copper challenge provides an energetically low cost detoxification mechanism. Histidine elevation may also provide a novel molecular biomarker of Cu II exposure in environmental situations.

译文

:高分辨率的1H NMR光谱对生物流体,细胞和组织提取物进行分析,可在不进行样品预处理的情况下对各种代谢物和有机化合物进行快速,无损的分析。我们已应用高分辨率1H NMR光谱技术研究了Cu II对分别在实验室和半田间条件下暴露的两种earth Eisenia andrei n = 78和Lumbricus rubellus n = 45的生化作用。最显着的代谢反应是动物体内​​内源性全身游离组氨酸的升高,这与半田野实验中铜暴露量的增加和总铜负荷的增加呈正相关。组氨酸在广泛的物理化学条件下形成热力学稳定的铜络合物,我们提出响应铜挑战的游离组氨酸的升高提供了一种能量低廉的排毒机理。在环境条件下,组氨酸的升高也可能提供Cu II暴露的新型分子生物标记。

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