1. Intravital microscopy of rabbit tenuissimus muscle microvasculature was used for in vivo studies of the role of endogenous nitric oxide (NO) in local vascular control. Derivatives of arginine were applied topically in order to modulate the formation of NO from L-arginine. 2. L-NG-monomethylarginine (L-NMMA) (10-100 microM), but not D-NG-monomethylarginine (D-NMMA), dose-dependently reduced microvascular diameters. The vasoconstriction induced by L-NMMA (100 microM) was prevented by pretreatment with L-arginine (1 mM) but not with D-arginine (1 mM). Intravenous infusions of L-arginine (300 mg kg-1) reversed the effect of L-NMMA (100 microM). L-Arginine or D-arginine applied topically at 1 mM per se had no effect on microvascular diameters. 3. Vasodilatation by acetylcholine (0.03-3 microM) was significantly inhibited by L-NMMA (100 microM), whereas vasodilatation by adenosine (0.1-100 microM) or sodium nitroprusside (100 nM) was not affected. 4. The hyperaemic response after tenuissimus muscle contractions induced by motor nerve stimulation was unaffected by the presence of L-NMMA (100 microM). 5. Aggregates of platelets and white blood cells were seen in venules during superfusion with L-NMMA (100 microM), but not with D-NMMA (100 microM). 6. Our results suggest that endogenous NO formed from L-arginine is a modulator of microvascular tone and platelet and white cell-vessel wall interaction in vivo. Nitric oxide does not, however, appear to play a role in the mediation of functional hyperaemia in this tissue.

译文

:1。兔腱鞘肌微脉管系统的活体显微镜用于体内研究内源性一氧化氮(NO)在局部血管控制中的作用。局部应用精氨酸衍生物以调节由L-精氨酸形成NO的过程。 2. L-NG-单甲基精氨酸(L-NMMA)(10-100 microM),而不是D-NG-单甲基精氨酸(D-NMMA),剂量依赖性地降低了微血管直径。 L-NMMA(100 microM)诱导的血管收缩可通过用L-精氨酸(1 mM)预处理而不是D-精氨酸(1 mM)来预防。静脉内注射L-精氨酸(300 mg kg-1)可逆转L-NMMA(100 microM)的作用。本身以1 mM局部应用的L-精氨酸或D-精氨酸对微血管直径没有影响。 3. L-NMMA(100 microM)显着抑制了乙酰胆碱(0.03-3 microM)的血管舒张作用,而腺苷(0.1-100 microM)或硝普钠(100 nM)的血管舒张作用未受到影响。 4.运动神经刺激引起的腱鞘肌收缩后的充血反应不受L-NMMA(100 microM)的影响。 5.在与L-NMMA(100 microM)融合时,小静脉中可见血小板和白细胞的聚集,但与D-NMMA(100 microM)融合时未见。 6.我们的结果表明,由L-精氨酸形成的内源性NO是体内微血管张力和血小板与白细胞-血管壁相互作用的调节剂。然而,一氧化氮似乎在该组织的功能性充血的介导中不起作用。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录