Age-related changes in primary lymphoid organs are well described. Less is known about age-related changes affecting peripheral lymphoid organs, although defects in old peripheral lymph nodes (pLNs) were recently described in both steady state and during viral infection. To address whether such pLN defects were intrinsic to old T cells or extrinsic (due to aging microenvironment), we employed heterochronic parabiosis. We found no age-related intrinsic or extrinsic barriers to T cell circulation and seeding of pLN, spleen, and bone marrow. However, heterochronic parabiosis failed to improve cellularity of old pLN, suggesting an environment-based limit on pLN cellularity. Furthermore, upon parabiosis, pLN of the adult partner exhibited reduced, old-like stromal and T cell cellularity, which was restored following separation of parabionts. Decay measurement of adult and old T cell subsets following separation of heterochronic parabionts delineated both T cell-intrinsic and environmental changes in T cell maintenance. Moreover, parabiotic separation revealed differences between CD4 and CD8 T cell subset maintenance with aging, the basis of which will require further investigation. Reasons for this asymmetric and subset-specific pattern of differential maintenance are discussed in light of possible age-related changes in lymph nodes as the key sites for peripheral T cell maintenance.

译文

:与淋巴管原发性器官的年龄相关的变化已被很好地描述。关于年龄相关变化影响外周淋巴器官的知之甚少,尽管最近已在稳定状态和病毒感染期间描述了旧的外周淋巴结(pLNs)的缺陷。为了解决此类pLN缺陷是旧T细胞固有的还是外部T细胞固有的(由于老化的微环境),我们采用了异时共生。我们没有发现年龄相关的内在或外在障碍对T细胞循环和pLN,脾脏和骨髓的播种。但是,异时生物共生不能改善旧pLN的细胞性,这表明基于环境的pLN细胞性受到限制。此外,在发生共生关系时,成年伴侣的pLN表现出减少的,老样的基质和T细胞细胞性,这在分离代谢物后得以恢复。异时生物体分离后,成年和老T细胞亚群的衰变测量描述了T细胞内在性和T细胞维持环境的变化。此外,共生生物分离揭示了CD4和CD8 T细胞亚群维持与衰老之间的差异,其基础将需要进一步研究。鉴于可能与年龄相关的淋巴结变化是外周T细胞维持的关键部位,讨论了这种差异维护的非对称和子集特定模式的原因。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录