Diffuse intrinsic pontine gliomas arise almost exclusively in children, and despite advances in treatment, the majority of patients die within 2 years after initial diagnosis. Because of their infiltrative nature and anatomic location in an eloquent area of the brain, most pontine gliomas are treated without a surgical biopsy. The corresponding lack of tissue samples has resulted in a limited understanding of the underlying genetic and molecular biologic abnormalities associated with pontine gliomas, and is a substantial obstacle for the preclinical testing of targeted therapeutic agents for these tumors. We have established a human glioma cell line that originated from surgical biopsy performed on a patient with a pontine glioma. To insure sustainable in vitro propagation, tumor cells were modified with hTERT (human telomerase ribonucleoprotein reverse transcriptase), and with a luciferase reporter to enable non-invasive bioluminescence imaging. The hTERT modified cells are tumorigenic in athymic rodents, and produce brainstem tumors that recapitulate the infiltrative growth of brainstem gliomas in patients.

译文

:弥漫性桥脑神经胶质瘤几乎仅在儿童中发生,尽管治疗有所进步,但大多数患者在初诊后2年内死亡。由于其浸润性和大脑雄辩区域的解剖位置,大多数桥脑神经胶质瘤无需手术活检即可治疗。组织样品的相应缺乏导致对与桥脑神经胶质瘤相关的潜在遗传和分子生​​物学异常的了解有限,并且是针对这些肿瘤的靶向治疗剂进行临床前测试的主要障碍。我们已经建立了人类脑胶质瘤细胞系,该细胞系源自对桥脑胶质瘤患者进行的手术活检。为了确保可持续的体外繁殖,使用hTERT(人端粒酶核糖核蛋白逆转录酶)和荧光素酶报道分子修饰肿瘤细胞,以实现非侵入性生物发光成像。 hTERT修饰的细胞在无胸腺啮齿动物中具有致瘤性,并产生脑干肿瘤,可概括患者脑干神经胶质瘤的浸润性生长。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录