BACKGROUND:Granulocyte apheresis is a safe and effective method for granulocyte collection. We present a five year experience (2006-2010) of the Blood Center, Faculty Hospital Ostrava, Czech Republic. Donor granulocyte transfusion is one treatment option for haemato-oncology patients with severe neutropenia complicated by bacterial/fungal infections unresponsive to standard antibiotic/antifungal treatment. In this study, we describe the experiences of the Blood Centre at the Faculty Hospital in Ostrava of granulocyte apheresis. METHODS AND RESULTS:A total of 149 granulocyte units were collected for 33 patients from the Department of Haemato-oncology, University Hospital Olomouc, over a 5-year period (2006-2010). Information on donor selection, laboratory screening, mobilization regimen and granulocyte yield was obtained and analyzed. All mandatory screening tests for infection markers, AB0 and Rh and abnormal erythrocyte antibodies were performed. The cytomegalovirus status of the donors was not investigated. Steroids were the only mobilization regimen used, and were generally well accepted. The mean granulocyte yield was 12.6×10(9)/per transfusion unit (range 5.4-30.3). All granulocyte concentrates were irradiated and transfused according to AB0 Rh compatibility within 24 h after collection. CONCLUSION:Based on our experience, granulocytapheresis is a safe and effective method for obtaining granulocytes but the yield can be significantly influenced by other variables. From the recipients' perspective, the use of donor granulocytes supports an effective therapeutic modality.

译文

背景:粒细胞单采是一种安全有效的收集粒细胞的方法。我们介绍了捷克共和国俄斯特拉发学院医院血液中心的五年经验(2006-2010)。供体粒细胞输注是严重中性粒细胞减少症并发细菌/真菌感染而对标准抗生素/抗真菌治疗无反应的血液肿瘤患者的一种治疗选择。在这项研究中,我们描述了粒细胞单采的俄斯拉发大学医院血液中心的经验。
方法与结果:在5年期间(2006-2010年),从Olomouc大学医院血液肿瘤科收集了149粒粒细胞单位,用于33例患者。获得并分析了有关供体选择,实验室筛查,动员方案和粒细胞产量的信息。进行了所有感染标志物,AB0和Rh和异常红细胞抗体的强制性筛查测试。没有研究捐赠者的巨细胞病毒状况。类固醇是唯一使用的动员方案,并且普遍被接受。平均粒细胞产量为12.6×10(9)/每输血单位(范围5.4-30.3)。收集后24小时内,按照AB0 Rh相容性对所有粒细胞浓缩物进行辐照和输血。
结论:根据我们的经验,粒细胞穿刺术是一种安全有效的获取粒细胞的方法,但产量会受到其他变量的显着影响。从接受者的角度来看,使用供体粒细胞支持有效的治疗方式。

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