BACKGROUND:Laboratory animal workers are at high risk of developing occupational allergy. Little is known about the relationship between levels of exposure and the risk of developing laboratory animal allergy. Since laboratory animal work comprises a large number of different-often short lasting-tasks, it is of interest to assess which activities are associated with high, low or intermediate levels of allergen exposure. OBJECTIVE:To develop and evaluate highly sensitive immunoassays in order to quantify rat and mouse urinary allergens in airborne dust sampled during short-lasting tasks. METHODS:Personal air dust samples were taken during full-shift periods as well as during specific tasks in seven laboratory animal facilities. Two sandwich enzyme immunoassays were developed, using rabbit antisera against rat and mouse urinary proteins. The rabbit antibodies were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting and compared with IgE antibodies from sensitized laboratory animal workers. RESULTS:The rabbit antibodies were highly specific for rat and mouse urinary proteins and reacted with all IgE-binding allergens in either urinary protein preparation. The assays for rat and mouse urine were very sensitive, with detection limits of 0.075 ng/mL. The coefficient of variation of the analysis was 12.9% for both assays. Animal caretakers appeared to experience the highest exposure to aeroallergens. A large variation in exposure within jobs was found, due to differences between tasks performed during the sampling day and the facility worked at. The highest exposure levels were found during removal of contaminated bedding from the cages. However, rat and mouse allergen exposure levels during this task varied enormously between facilities, 1.1-158 ng eq/m3 and 0.63-2000 ng eq/m3, respectively. CONCLUSION:Both sandwich immunoassays are highly specific and sensitive and are able to identity tasks of relatively short duration with high, medium and low exposure to airborne rat and mouse urinary allergens.

译文

背景:实验室动物工作者有发展职业过敏的高风险。关于暴露水平与发生实验动物过敏的风险之间的关系知之甚少。由于实验室动物工作包括大量不同的,通常是短期的任务,因此评估哪些活动与高,低或中等水平的过敏原暴露相关是很有意义的。
目的:开发和评估高度敏感的免疫分析方法,以定量在短期任务中采样的空气中粉尘中的大鼠和小鼠尿液过敏原。
方法:在全班工作期间以及在特定任务期间,在七个实验室动物设施中采集了个人空气尘埃样本。使用针对大鼠和小鼠尿蛋白的兔抗血清,开发了两种夹心酶免疫测定法。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和免疫印迹分析兔抗体,并将其与来自敏化实验动物工作者的IgE抗体进行比较。
结果:兔抗体对大鼠和小鼠的尿蛋白具有高度特异性,并与两种尿蛋白制剂中的所有IgE结合过敏原反应。大鼠和小鼠尿液的测定非常敏感,检出限为0.075 ng / mL。两种测定的分析变异系数均为12.9%。动物看护者似乎经历了最高的空气过敏原暴露。由于在采样日执行的任务与所使用的设施之间存在差异,因此发现工作中的暴露程度存在很大差异。从笼子中取出受污染的被褥时发现了最高暴露水平。但是,在此任务期间,大鼠和小鼠的过敏原暴露水平在设施之间的差异很大,分别为1.1-158 ng eq / m3和0.63-2000 ng eq / m3。
结论:这两种夹心免疫测定法都具有高度特异性和敏感性,能够识别持续时间相对较短,高,中和低暴露于机载大鼠和小鼠尿液变应原的任务。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录