The present study investigated the effect of resveratrol on the electrophysiology and insulin secretion of pancreatic beta cells, and examined resveratrol-induced alterations in insulin levels and plasma glucose of normal and streptozotocin-induced diabetic rats. Whole-cell voltage clamp study in the MIN6 cell, a mouse beta cell line, revealed that resveratrol significantly inhibited ATP-sensitive K(+) current at 3 micromol/l, and voltage-gated K(+) currents at 30 micromol/l. Ca(2+)-activated K(+) current was activated by resveratrol at 100 micromol/l. In MIN6 cells stained with membrane potential dye DiBAC(4)(5), resveratrol markedly depolarized membrane potential at the concentrations of 3-100 micromol/l. Insulin secretion was increased in the presence of resveratrol in MIN6, Hit-T15, and RIN-m5F cells. Resveratrol (3 mg/kg, i.p.) increased insulin secretion associated with a lowering in plasma glucose in normal rats, but not in streptozotocin-diabetic rats within the initial 60 min. In conclusion, resveratrol can act as an insulin-secretagogue through I(KATP) and I(KV) inhibition which can contribute to plasma glucose lowering effect in normal rats.

译文

:本研究调查了白藜芦醇对胰腺β细胞电生理和胰岛素分泌的影响,并研究了白藜芦醇诱导的正常和链脲佐菌素诱导的糖尿病大鼠胰岛素水平和血浆葡萄糖的变化。在MIN6细胞(一种小鼠​​β细胞系)中进行全细胞电压钳研究,发现白藜芦醇在3微摩尔/升时显着抑制ATP敏感的K()电流,在30微摩尔/升时抑制电压门控的K()电流。 Ca(2)激活的K()电流被白藜芦醇以100 micromol / l激活。在用膜电位染料DiBAC(4)(5)染色的MIN6细胞中,白藜芦醇在3-100 micromol / l的浓度下可显着去极化膜电位。在MIN6,Hit-T15和RIN-m5F细胞中存在白藜芦醇时,胰岛素分泌增加。在正常大鼠中,白藜芦醇(3 mg / kg,i.p.)在最初60分钟内胰岛素分泌增加与血浆葡萄糖降低有关,但在链脲佐菌素-糖尿病大鼠中却没有。总之,白藜芦醇可以通过I(KATP)和I(KV)抑制作用起胰岛素分泌的作用,这可以促进正常大鼠血浆葡萄糖的降低作用。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录