Isolated rat adipose tissue-derived stromal cells (rATSCs) contain pluripotent cells that can be differentiated into a variety of cell lineages, including neural cells. Recent work has shown that ATSCs can make neurosphere-like clumps and differentiate into neuron-like cells expressing neuronal markers, but their therapeutic effect is unclear. Here we report that intravenous infusion of oligodendrocyte precursor cells (OPCs) derived from rATSC autograft cells sources improve motor function in rat models of spinal cord injury (SCI). After 4-5 weeks, transplanted rATSC-OPC cells survived and migrated into the injured region of SCI very efficiently (30-35%) and migrated cells were partially differentiated into neurons and oligodendrocyte. Also, we found some of the engrafted OPCs migrated and integrated in the kidney, brain, lung, and liver through the intravenous system. Behavioral analysis revealed the locomotor functions of OPC-autografted SCI rats were significantly restored. Efficient migration of intravenously engrafted rATSC-OPCs cells into SCI lesion suggests that SCI-induced chemotaxic factors facilitate migration of rATSC-OPCs. Here, we verified that engrafted rATSCs and SCI-induced chemotaxic factors indeed play an important role in proliferation, migration, and differentiation of endogeneous spinal cord-derived neural progenitor cells in the injured region. In transplantation paradigms, the interaction between engrafted rATSC-OPCs and endogeneous spinal cord-derived neuronal progenitor cells will be important in promoting healing through fate decisions, resulting in coordinated induction of cell migration and differentiation.

译文

:分离的大鼠脂肪组织来源的基质细胞(rATSC)包含多能细胞,可以分化为多种细胞谱系,包括神经细胞。最近的工作表明,ATSCs可以使神经球样团块并分化为表达神经元标记物的神经元样细胞,但其治疗效果尚不清楚。在这里,我们报道静脉输注源自rATSC自体移植细胞来源的少突胶质前体细胞(OPC)可改善大鼠脊髓损伤(SCI)模型的运动功能。 4-5周后,移植的rATSC-OPC细胞存活并非常有效地(30-35%)迁移至SCI的受损区域,并且迁移的细胞部分分化为神经元和少突胶质细胞。此外,我们发现一些植入的OPC通过静脉系统迁移并整合在肾脏,脑,肺和肝脏中。行为分析表明,OPC自体移植SCI大鼠的运动功能得到了显着恢复。静脉移植的rATSC-OPCs细胞向SCI病变的有效迁移表明,SCI诱导的化学趋化因子促进了rATSC-OPCs的迁移。在这里,我们验证了移植的rATSCs和SCI诱导的趋化因子确实在受损区域内源性脊髓来源的神经祖细胞的增殖,迁移和分化中起着重要作用。在移植范例中,移植的rATSC-OPC与内源性脊髓源性神经元祖细胞之间的相互作用对于通过命运决定促进愈合,导致细胞迁移和分化的协同诱导非常重要。

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