The nature of the primary genetic defects in ob/ob and db/db mice are unknown. Both the obese (ob) and diabetes (db) mutations produce similar, multicomponent obese-hyperinsulinemic syndromes when maintained in the same strain of mouse. In an attempt to find differences between these mutations in neuroendocrine function affecting the islets of Langerhans or the pituitary, tissue content of four neuropeptides that are known to be capable of influencing the rate of insulin secretion was examined in obese (ob/ob) and diabetes (db/db) mice. In the first study, C57BL/6Job/ob and control males were studied at 3, 4, and 11 weeks of age. In the second study, db/db mice of both sexes and two inbred strains (C57BL/6J and C57BL/KsJ), which differ markedly in the severity of expression of the diabetes phenotype, were studied at 3 weeks of age, before the development of hyperglycemia and secondary consequences thereof. Immunoreactive peptides were measured in acetic acid extracts of pancreas and pituitary. No differences between male ob/ob and db/db mice of the C57BL/6J strain were found. Marked sex differences in lean control mice were found at 3 weeks of age in pancreatic Met-enkephalin-LI and galanin-LI (with two- to threefold higher content in males). Low pancreatic content (50% to 70% lower than in control mice) of galanin-LI, Met-enkephalin-LI and Leu-enkephalin-LI was associated with hyperinsulinemia in male B6 ob/ob and db/db mice at 3 weeks of age, though not in B6 db/db females and not in BKs db/db mice of either sex.(ABSTRACT TRUNCATED AT 250 WORDS)

译文

:ob / ob和db / db小鼠的主要遗传缺陷的性质尚不清楚。肥胖(ob)和糖尿病(db)突变在同一小鼠品系中均会产生相似的多成分肥胖-高胰岛素血症综合征。为了发现影响兰格罕氏岛或垂体胰岛的神经内分泌功能的这些突变之间的差异,在肥胖和肥胖(ob / ob)和糖尿病患者中,对四种已知能够影响胰岛素分泌速率的神经肽的组织含量进行了检查。 (db / db)小鼠。在第一个研究中,研究了C57BL / 6Job / ob和对照男性在3、4和11周的年龄。在第二项研究中,在发育前的3周龄研究了性别和两种自交系(C57BL / 6J和C57BL / KsJ)的db / db小鼠,它们在糖尿病表型的表达严重程度上有显着差异。高血糖及其继发后果。在胰腺和垂体的乙酸提取物中测量了免疫反应性肽。在C57BL / 6J株的雄性ob / ob和db / db小鼠之间未发现差异。在瘦瘦对照小鼠中,在三周龄的胰腺Met-脑啡肽-LI和甘丙肽-LI中发现明显的性别差异(雄性含量高2至3倍)。在3周龄的雄性B6 ob / ob和db / db小鼠中,甘丙肽-LI,Met-脑啡肽-LI和亮-脑啡肽-LI的胰腺含量低(比对照小鼠低50%至70%)与高胰岛素血症相关。年龄,但不是在B6 db / db雌性中,也不是在BK s db / db两种性别的小鼠中。(摘要以250字截断)

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