A novel marine, Gram-negative, rod-shaped bacterium, designated strain N9(T), was isolated from a water sample of the sea shore at Visakhapatnam, Andhra Pradesh (India). Strain N9(T) was found to be positive for oxidase and catalase activities. The fatty acids were found to be dominated by C(16:0), C(18:1) ω7c and summed in feature 3 (C(16:1) ω7c and/or C(16:1) ω6c). Strain N9(T) was determined to contain Q-10 as the major respiratory quinone and phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, two phospholipids and four unidentified lipids as polar lipids. The DNA G+C content of the strain N9(T) was found to be 63 mol%. 16S rRNA gene sequence analysis indicated that Rhodobacter sphaeroides, Rhodobacter johrii, Pseudorhodobacter ferrugineus, Rhodobacter azotoformans, Rhodobacter ovatus and Pseudorhodobacter aquimaris were the nearest phylogenetic neighbours, with pair-wise sequence similarities of 95.43, 95.36, 94.24, 95.31, 95.60 and 94.74 %, respectively. Phylogenetic analysis showed that strain N9(T) formed a distinct branch within the family Rhodobacteraceae and clustered with the clade comprising species of the genus Pseudorhodobacter, together with species of the genera Roseicitreum, Roseinatronobacter, Roseibaca and Rhodobaca. Species of the genus Pseudorhodobacter are phylogenetically close with a 16S rRNA gene sequence dissimilarity of 5.9-7.3 % (92.7-94.1 % similarity). Based on the above-mentioned phenotypic characteristics and on phylogenetic inference, strain N9(T) is proposed as a representative of a new genus and a novel species of the family Rhodobacteraceae as Albirhodobacter marinus gen. nov., sp. nov. The type strain of Albirhodobacter marinus is N9 (= MTCC 11277(T) = JCM 17680(T)).

译文

:从印度安得拉邦维沙卡帕特南的海边水样中分离出一种新型海洋革兰氏阴性棒状细菌,命名为菌株N9(T)。发现菌株N9(T)对于氧化酶和过氧化氢酶活性是阳性的。发现脂肪酸主要由C(16:0),C(18:1)ω7c所占,并累加到特征3中(C(16:1)ω7c和/或C(16:1)ω6c)。确定菌株N9(T)含有Q-10作为主要的呼吸醌和磷脂酰乙醇胺,磷脂酰甘油,两个氨基磷脂,两个磷脂和四个未鉴定的脂质作为极性脂质。发现菌株N9(T)的DNA G C含量为63mol%。 16S rRNA基因序列分析表明球形球菌,拟南假单胞菌,费氏假单胞菌,偶氮假单胞菌,卵形假单胞菌和无水假单胞菌是最接近的系统发育邻居,成对配对的相似性分别为95.43、95.36、94.24、95.43、94.24、94.24、94.24。分别。系统发生学分析表明,菌株N9(T)在红细菌科内形成了一个独特的分支,并与包含假单胞菌属物种,蔷薇科,迷迭香属,罗塞巴卡属和罗多巴科属的枝条聚集在一起。假单胞菌属的物种在系统发育上很接近,其16S rRNA基因序列的相似性为5.9-7.3%(相似性为92.7-94.1%)。基于上述表型特征和系统发育推论,提出菌株N9(T)作为红藻纲科的一个新属和新物种,作为海藻白纹杆菌属的代表。十一月,sp。十一月海水杆菌的类型菌株为N9(= MTCC 11277(T)= JCM 17680(T))。

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